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Atip Lawanprasert1, Harminder Singh1, Sopida Pimcharoen1

  • 1Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA.

Nature communications
|December 31, 2024
PubMed
概括

研究人员开发了新的化工配方,以稳定生物药物和疫苗,而无需冷藏. 这一创新可能会消除对冷链物流的需求,改善可访问性并降低基本的基于蛋白质的治疗方法的成本.

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...