用干扰度光显微镜实时监测菌体悬浮物用于个性化菌体治疗
Benjamine Lapras1,2, Camille Merienne3, Emma Eynaud3
1Pharmacy Department, Hospices Civils de Lyon, Hôpital E. Herriot, Plateforme FRIPHARM, 69437, Lyon, France. benjamine.lapras@chu-lyon.fr.
Scientific reports
|December 31, 2024
概括
干涉度光显微镜 (ILM) 提供了一种快速的方法来控制菌体治疗悬浮物的质量. 该技术将颗粒度与传染性位数相关联,有助于评估菌体纯度和聚合.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 纳米技术纳米技术
背景情况:
- 菌体疗法是抗生素的有希望的替代品,用于对抗耐药细菌.
- 目前用于评估菌体悬浮的方法在速度,特异性和聚合检测方面面临挑战.
- 高度的净化菌体对于有效的治疗剂量至关重要.
研究的目的:
- 评估干扰度光显微镜 (ILM) 作为菌体悬浮的快速质量控制工具.
- 评估ILM对粒子度,大小分布和同质性的表征能力.
- 将ILM与其他用于菌体制剂中聚合物检测的技术进行比较.
主要方法:
- 应用ILM净化抗黄金葡萄球菌 (Staphylococcus aureus myovirus) 菌素悬浮物.
- 通过ILM测量的颗粒度与菌体感染 titre (斑块形成单位) 的相关性.
- 将ILM与动态光散射,电泳性移动性和聚合物检测的紫外线/可见光谱技术进行比较.
主要成果:
- 在悬浮剂≥3 × 108 PFU/mL的颗粒度和传染性位数之间发现了强烈的线性相关性 (R2 > 0.97).
- ILM有效地表征了粒子度,大小分布和均性,净化改善了这些参数.
- 与其他测试方法相比,ILM表现出优异的聚合检测.
结论:
- ILM作为一种有价值的,快速的质量控制工具,用于缩的菌体悬浮,补充传统的定位方法.
- 该技术可以大致估计传染性标位,并评估菌素制剂的均性.
- 虽然ILM对小菌体或低度有局限性,但它显示出优化菌体疗法生产的巨大潜力.
相关概念视频
01:30Lytic Cycle of Bacteriophages
70.3K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.3K
00:43Lysogenic Cycle of Bacteriophages
61.8K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
61.8K