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在甲状腺癌中,ACTN1促进细胞入侵,迁移和EMT,并与免疫透有关

Song Chen¹, Xue Luo², Wentai Wang³

¹School of Clinical Medicine, GuiZhou Medical University, Guiyang, Guizhou, China.

Scientific reports

|December 31, 2024

概括

在甲状腺癌 (THCA) 中,α-actin-1 (ACTN1) 被上调调节,促进瘤进展和转移. 向ACTN1可能通过抑制PI3K/AKT/mTOR通路来提供一种新的THCA治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

阿尔法-动因-1 (ACTN1) 是一种细胞骨蛋白质,与瘤进展有关.
在甲状腺癌 (THCA) 中ACTN1的作用仍然在很大程度上未被描述.

研究的目的:

研究THCA中ACTN1的表达和生物功能.
阐明ACTN1在THCA进展中的潜在分子机制.

主要方法:

基因表达数据的生物信息学分析.
定量实时PCR (qRT-PCR),免疫组织化学和西部抹杀.
在体外和体外功能测定 (细胞增殖,迁移,入侵,EMT).
对PI3K/AKT/mTOR信号通路的研究.

主要成果:

在THCA组织中,ACTN1显著上调,与不良预后因素相关.
过度表达ACTN1增强了THCA细胞的增殖,迁移,入侵和上皮-介质细胞过渡 (EMT).
通过ACTN1 knockdown抑制了这些恶性行为,并减少了PI3K/AKT/mTOR通路的激活.
在ACTN1淘汰细胞中PI3K激活后恢复入侵和迁移证实了途径的参与.

结论:

ACTN1是THCA进展,入侵和转移的关键调节者.
通过激活PI3K/AKT/mTOR通路,ACTN1促进THCA恶性瘤的发生.
ACTN1代表了THCA的潜在治疗标和预后生物标志物.

关键词:

在ACTN1中,ACTN1是 ACTN1 的一个组成部分.攻击性亚型攻击性亚型皮质 - 介质细胞过渡 (EMT)预测预后预测预测甲状腺癌是什么?甲状腺癌是什么?瘤免疫瘤免疫瘤转移的发生.

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