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相关概念视频

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.6K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

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Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
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Viral Structure00:56

Viral Structure

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Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
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Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

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The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
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核磁共振光谱用于研究病毒结构.

José L Neira1,2

  • 1IDIBE, Universidad Miguel Hernández, Elche, Alicante, Spain. jlneira@umh.es.

Sub-cellular biochemistry
|December 31, 2024
PubMed
概括

核磁共振 (NMR) 光谱学揭示了病毒蛋白质结构和动态. 这种技术对于理解病毒蛋白相互作用,形状变化和无序蛋白质至关重要,有助于病毒生命周期研究.

关键词:
会议会议会议会议会议会议会议会议会议会议有约束力的约束力卡普西德 (Capsid) 是一种状的鱼.化学转移是指化学转移的发生.规范性变化 规范性变化合常量是一个合常量.动力学 动力学 动力学电子类磁共振 (EPR) 是一种电子类磁共振.这是一个平衡的平衡.灵活性 灵活性 灵活性交换气的交换方式脂质 脂质 脂质 是一种膜膜是一种膜.核磁共振 (NMR) 是一种核磁共振技术.核酸是核酸中的一种.蛋白质与核酸的相互作用蛋白质与蛋白质之间的相互作用固态硬件是一种固态硬件.溶液结构结构的解决方法没有折叠的蛋白质.病毒 病毒 病毒

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科学领域:

  • 生物物理学的生物物理.
  • 结构生物学 结构生物学
  • 病毒学 病毒学

背景情况:

  • 核磁共振 (NMR) 光谱是一种分析分子结构和动态的强大技术.
  • 它在病毒学中广泛应用于研究病毒蛋白,核酸及其相互作用.

研究的目的:

  • 突出NMR光谱在病毒学中的应用.
  • 为了证明NMR在阐明病毒蛋白结构,动态和相互作用方面的能力.

主要方法:

  • 使用核磁共振 (NMR) 光谱在液态和固态.
  • 为了结构研究,用X射线晶体学和电子显微镜来补充NMR.

主要成果:

  • 核磁共振使病毒蛋白域及其相互作用的"自下而上"结构确定成为可能.
  • 它揭示了病毒蛋白和核酸的结构变化和动态平衡.
  • 核磁共振 (NMR) 提供了对内在无序的病毒蛋白和热力学结合参数的原子层次洞察.

结论:

  • 核磁共振光谱对了解病毒蛋白结构,动态和功能起着重要作用.
  • 它与其他技术的整合增强了复杂病毒系统的研究.
  • 核磁共振对于描述病毒组件及其在感染和组装过程中的行为至关重要.