相关实验视频
Updated: Jun 4, 2025

10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
4.8K
杂交外基因因子通过在核酸水平上合转录启动和剪接而进化
Steven T Mick1, Christine L Carroll1, Maritere Uriostegui-Arcos1
1Biology Department, Boston University, 24 Cummington Ave., Boston, 02215, USA.
Nucleic acids research
|December 31, 2024
概括
混合外子,其功能是第一外子和内部外子,显示转录启动和RNA剪接之间紧密的合. 这些外因子通常在它们的3'连接部位附近启动转录,揭示了复杂的基因调节.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 传统上,前子被分为第一个,内部或最后一个,每个都有不同的调节机制.
- 已经确定了混合型外子,它们在不同的转录中作为终端外子和内部外子发挥作用.
- 了解混合元的调节机制对于理解基因表达复杂性至关重要.
研究的目的:
- 使用深度学习来剖析控制转录启动和杂交外子拼接联合调节的序列特征.
- 在人体组织中识别和表征杂交的第一内部外因子.
- 调查杂交外体的进化起源和调节作用.
主要方法:
- 利用可解释的深度学习管道来分析混合元的序列特征.
- 采用了来自人体组织的ENCODE数据来识别杂交第一内部外体.
- 分析了染色质状态,拼接点强度和促进物元素丰富度.
主要成果:
- 在人体组织中鉴定了8万个混合的第一个内部外因子.
- 这些表原体表现出放松的染色体状态,使基因体内的转录启动成为可能.
- 转录起点通常以3'拼接位为中心,表明结合的转录-拼接.
- 确定了两个子类别:那些类似于内部外显子 (强3'拼接位) 和那些类似于第一个外显子 (促销元件) 的子类别.
- 进化分析揭示了人类杂交外体中3'拼接位/外体或促进元件的增加.
结论:
- 混合异构体代表了一类具有复杂序列特征的重要调节元件.
- 证明了转录启动和RNA剪接之间强烈的,以前未被承认的联系.
- 这些发现为基因表达和外体定义的监管格局提供了新的见解.
相关概念视频
RNA Splicing
56.0K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.0K
Alternative RNA Splicing
21.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.0K
Exon Recombination
3.5K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.5K
Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
Chromatin Structure Regulates pre-mRNA Processing
6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
6.9K
Pre-mRNA Processing: Modification of pre-mRNA Ends
9.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.2K

