全基因组再测序揭示了Kele猪的原相关基因
Yu Dan Zhang1,2,3, Wei Yuan3, Huan Bi4
1Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, Guizhou Province, China.
PloS one
|December 31, 2024
概括
全基因组再测序在Kele猪中发现了与原相关的基因和SNP标记物. 这些发现支持这种品种改善原特征的分子选择.
科学领域:
- 基因组学就是基因组学.
- 动物遗传学动物遗传学
- 分子生物学分子生物学
背景情况:
- 凯莱猪具有宝贵的原特性.
- 全基因组重新排序 (WGR) 为识别遗传标记提供了一个强大的工具.
- 了解原蛋白基因调节对于品种改进至关重要.
研究的目的:
- 为了验证通过WGR在Kele猪中识别的原特异单核酸多态 (SNP) 突变位.
- 在Kele猪中发现新的原相关基因.
- 建立分子选择策略的基础.
主要方法:
- 全基因组再测序 (WGR) 用于选候选原相关基因.
- 进行了基因注释,KEGG和GO丰富分析.
- 四个关键基因 (COL9A1,COL6A5,COL4A3,COL4A4) 被选择进行进一步研究.
- 桑格测序验证了14个随机选择的SNP网站.
- 通过RT-qPCR评估了不同Kele猪组织中的基因表达水平.
主要成果:
- WGR产生了241.04Gb的高质量的清洁数据 (Q30 ≥93.96%) 和平均覆盖深度为9.04×.
- 分析发现了许多高,中,低影响SNP和INDEL突变部位,影响蛋白质功能和特性.
- 通过RT-qPCR检测证实,皮肤中的COL9A1和COL6A5,以及脏组织中的COL4A4和COL4A3的表达更高.
- 桑格测序验证了由WGR识别的14个选择的SNP突变位点的准确性.
结论:
- 总共有307个与原相关的基因被确定,包括COL9A1,COL6A5,EP300,SOS2和EPO.
- 特定的原基因 (COL9A1,COL6A5,COL4A3,COL4A4) 显示出显著的组织特异性表达模式.
- 来自WGR的验证SNP标记器为分析Kele猪原蛋白特征提供了可靠的基础.
- 这些发现有助于在未来对Kele猪进行研究和开发,以提高其原特性.
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