聚糖中的共价修饰通过TLR4-介导的MAPK/NF-κB信号通路增强免疫调节活动
Aijuan Bao1, Zhangkun Wei1, Meiting Bai1
1College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China.
Journal of agricultural and food chemistry
|December 31, 2024
概括
用改性豆 (SeLBG) 通过激活关键信号通路,显著增强免疫反应. 这种富含Se的成分显示出各种功能应用的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- (Se) 是一种重要的微量元素,具有强大的免疫调节特性,受其多样化的价值状态和代谢途径的影响.
- 豆 (LBG) 是一种银河甘,作为修饰的功能性多糖体载体.
研究的目的:
- 通过使用深溶解剂 (DES) 合成和表征共价改性豆 (SeLBG).
- 与LBG和各种化合物相比,评估SeLBG的免疫调节作用.
- 阐明潜在的分子机制,包括信号通路和受体相互作用.
主要方法:
- 使用DESs对LBG进行化,以产生具有不同含量的SeLBGs.
- 通过细胞活力,细胞化,细胞因子分泌和蛋白质表达测试来评估免疫调节活性.
- 分子对接,分子动力学模拟和受体抑制剂策略来研究TLR激活.
主要成果:
- 与原生LBG和化合物 (Se-Met,Na2SeO3,SeNPs) 相比,SeLBG在等效度下表现出优越的免疫调节活性.
- SeLBG激活了MAPK和NF-κB信号通路,从而提高了细胞活力,细胞活性和细胞因子分泌.
- 分子模拟表明,SeLBG有可能诱导类似收费受体 (TLR) 的二分化和激活,由抑制剂研究证实,显示增强的TLR2/4识别.
结论:
- 共价改性豆 (SeLBG) 具有增强的免疫调节能力.
- 观察到的效应是通过激活MAPKs,NF-κB和TLR2/4信号通路进行的.
- SeLBG代表了一个有前途的功能成分,在免疫健康和相关领域有潜在的应用.
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