植物黄酸的一氧化碳释放活动
Lucie Muchová1, Mária Šranková1, Sriram Balasubramani1
1Institute of Medical Biochemistry and Laboratory Diagnostics, and 4th Department of Internal Medicine, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, Na Bojišti 3, Prague 2 12108, Czech Republic.
Journal of agricultural and food chemistry
|December 31, 2024
概括
像奎尔丁这样的黄类化合物可以在暴露在光线下释放一氧化碳 (CO). 这种光释放的CO提供了对细胞损伤的保护作用,并表现出抗增殖性质,暗示了治疗潜力.
科学领域:
- 生物化学 生物化学
- 摄影化学的使用.
- 细胞生物学 细胞生物学
背景情况:
- 黄酸是植物衍生化合物,具有已知的健康益处,包括抗氧化和抗炎性质.
- 一氧化碳 (CO) 是一种新兴的信号分子,具有重要的生物作用.
- 作为可光激活的CO释放分子 (CORM),黄类药物的潜力在很大程度上仍未被探索.
研究的目的:
- 在生理条件下,研究氨酸和2,3-脱水氨酸作为可光激活的CORM.
- 为了评估这些黄素释放的二氧化碳的生物效应.
- 探索基于黄类的CORM的治疗影响.
主要方法:
- 在酸盐缓冲盐溶液 (PBS) 中直接光解和敏感光氧化奎尔丁和2,3-脱西利宾.
- 使用化学产量量量化释放的一氧化碳 (CO).
- 评估细胞反应,包括毒性,线粒体呼吸,超氧化物产生和细胞循环进展,以应对光释放的CO.
主要成果:
- 直接照射的黄类化合物释放的二氧化碳,化学产量高达0.23等值.
- 单一氧气显著增强了CO的释放,实现了接近统一的产量.
- 照相释放的CO减轻了高醇诱导的细胞毒性,恢复了线粒体功能,降低了超氧化物水平,并通过影响细胞周期阶段诱导了抗增殖效应.
结论:
- 奎尔塞丁和2,3-脱西利宾作为有效的可光激活的CORM.
- 从这些黄类化合物中释放的光氧化碳显示出显著的细胞保护和抗增殖活性.
- 这些发现表明,基于黄类的CORM在化学预防和治疗中的潜在应用,以及在植物生理学中的作用.
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