白色,柔软和排泄和红色,坚硬和非排泄的猪肉之间的蛋白质乳酸化差异
Chao Ma1, Wangang Zhang1, Lujuan Xing1
1State Key Laboratory of Meat Quality Control and Cultured Meat Development, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
由p300/cAMP响应元素结合蛋白 (CBP) 调节的蛋白质乳化,在白,柔软和排泄性 (PSE) 和红色,坚硬和非排泄性 (RFN) 猪肉之间存在显著差异. 这些发现为PSE猪肉发展提供了新的见解.
科学领域:
- 肉类科学 肉科学
- 生物化学 生物化学
- 死亡后的肌肉新陈代谢
背景情况:
- 白,柔软和排泄 (PSE) 猪肉是猪肉行业的一个主要缺陷.
- 了解PSE猪肉发育的生物化学基础对于质量改善至关重要.
研究的目的:
- 调查PSE中的蛋白质乳酸和其调节剂与红色,坚硬和非排泄性 (RFN) 猪肉.
- 探索E1A结合蛋白p300 (p300) 和cAMP反应元素结合蛋白 (CBP) 在死后肌肉乳化中的作用.
主要方法:
- 在死后1小时的PSE和RFN猪肉中比较糖原和乳酸盐水平.
- 蛋白质乳酸和p300/CBP水平的量化.
- 免疫光显微镜以确定p300/CBP和乳酸蛋白的局部和同局部.
主要成果:
- 与RFN猪肉相比,PSE猪肉呈现较低的糖原和较高的乳酸盐水平.
- 在PSE猪肉中观察到较高的p300/CBP和蛋白质乳酸化水平.
- 在死后的肌细胞中,p300/CBP和乳酸蛋白共同定位,表明p300/CBP是关键调节剂.
结论:
- 在PSE和RFN猪肉之间,蛋白质乳酸化水平显著不同.
- p300/CBP是死后肌肉中蛋白质乳酸化的关键调节剂.
- 蛋白质乳化可能在调节死后肌肉生化代谢和PSE猪肉质量方面发挥作用.
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