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相关概念视频

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
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与COVID-19严重程度相关的miRNA模式表达的变化

Paola B Zárate-Segura1, Macario Martínez-Castillo2, Aarón Paris Garduño-Gutiérrez3

  • 1Laboratorio de Medicina Traslacional, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico; pzarates@ipn.mx.

In vivo (Athens, Greece)
|December 31, 2024
PubMed
概括

在严重的COVID-19患者中,特定的microRNAs (miRNAs) 过度表达. 这些miRNAs,包括miR-490-3p,可能作为预测2019年冠状病毒疾病临床过程的潜在生物标志物.

关键词:
在 COVID-19 疫情中,miR-195-5p-5p的使用情况.在 miR-431-5p 中使用.在 miR-454-3p 中使用.在 miR-490-3p 中使用.严重程度的严重程度.

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科学领域:

  • 分子生物学分子生物学
  • 病毒学 病毒学
  • 遗传学 是一个遗传学.

背景情况:

  • 新冠病毒病2019 (COVID-19) 呈现出各种不同的临床症状.
  • 主体微RNAs (miRNAs) 可以影响COVID-19的进展和并发症.
  • 了解像miRNA表达这样的宿主因素对于管理严重的SARS-CoV-2感染至关重要.

研究的目的:

  • 在严重的COVID-19患者中调查内源性miRNA的表达模式.
  • 为了比较80个严重的COVID-19病例和健康对照之间的miRNA表达.
  • 为了确定与严重的SARS-CoV-2感染相关的特定miRNAs.

主要方法:

  • 使用TaqMan低密度阵列进行miRNA表达查.
  • 通过RT-qPCR对特定的miRNA表达 (miR-490-3p,miR-195-5p,miR-454-3p,miR-431-5p) 的验证.
  • 在分析用于目标识别和路径预测.

主要成果:

  • 与对照组相比,四种miRNAs (miR-490-3p,miR-195-5p,miR-454-3p,miR-431-5p) 在严重的COVID-19患者中显著过度表达.
  • 在男性和女性COVID-19患者中,miR-490-3p显示过度表达.
  • 这些发现突显了严重的SARS-CoV-2感染中特定的miRNA失调.

结论:

  • 特定的miRNAs在严重的COVID-19中表现出改变的表达.
  • 这些过度表达的miRNAs可以作为预测COVID-19临床结果的潜在生物标志物.
  • 对miRNA作用的进一步研究可以为2019年严重的冠状病毒疾病的治疗策略提供信息.