在CA2金字塔细胞和帕尔瓦胺表达细胞上的围神经网络不同调节海马体依赖记忆的记忆
Georgia M Alexander1, Viktoriya D Nikolova2, Tristan M Stöber3,4
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, Division of Intramural Research, National Institute of Health, Research Triangle Park, North Carolina 27713.
概括
围绕CA2神经元的周围神经元网络 (PNN) 损害了社会记忆,而帕瓦胺 (PV) 神经元上的神经网络则影响了恐惧记忆. 这项研究揭示了PNN在不同类型的大脑细胞中对记忆调节的不同作用.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 行为科学 行为科学
背景情况:
- 围神经网络 (PNN) 是细胞外矩阵,调节突触可塑性和记忆.
- 目前的方法,如ondroitinase降解缺乏细胞类型特异性的PNN功能.
- 在特定的神经元群体上解开PNN作用对于理解大脑功能至关重要.
研究的目的:
- 调查PNN在CA2金字塔细胞与PV内部神经元在调节行为的不同作用.
- 为PNN研究生成和分析细胞类型特定的aggrecan淘汰小鼠模型.
主要方法:
- 生成的Amigo2-Cre;Acan-floxed (Amigo2 Acan KO) 和PV-Cre;Acan-floxed (PV Acan KO) 小鼠,分别从CA2或PV细胞中删除关键PNN蛋白质aggrecan.
- 评估社会,恐惧和空间记忆,以及逆向学习,在雄性和雌性KO和野生类型小鼠中.
- 在Amigo2 Acan KO小鼠中研究了潜在的社会记忆缺陷的神经电路机制.
主要成果:
- Amigo2 Acan KO小鼠在社交记忆和逆向学习方面表现出缺陷.
- 在PV Acan KO小鼠中,情境恐惧记忆受损.
- Amigo2 Acan KO小鼠对CA2的超乳母细胞核 (SuM) 输入减少,缺乏与社会新奇相关的神经反应.
结论:
- 在CA2金字塔细胞和PV内部神经元上的PNN在依赖海马体的记忆中扮演独立的,细胞类型特定的角色.
- CA2 PNN对于通过SuM输入处理社会新性至关重要.
- 这项研究为PNN在不同神经元电路中的特定功能提供了新的见解.
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