Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

12.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
12.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Upconversion particle-based optical tweezers for sensing applications.

Nature protocols·2026
Same author

Moisture-Light Harvesting Enhanced Hydrovoltaic Electric Generation.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Photoenergy harvesting by ammonium molybdate soft hydrogel drops.

Light, science & applications·2025
Same author

Anomalously Large Luminescence Modulation Induced by Trace Lanthanide Impurities in Alloyed Upconversion Nanocrystals.

ACS nano·2025
Same author

Probing the Nanonewton Mitotic Cell Deformation Force by Ion-Resonance-Enhanced Photonics Force Microscopy.

Nano letters·2024
Same author

Miniaturized on-chip spectrometer enabled by electrochromic modulation.

Light, science & applications·2024
Same journal

Bi-layer photonic random meta-composite for cryogenic thermal control by ultra-broadband scattering matched reflectance.

Light, science & applications·2026
Same journal

Interferometric scattering for optical tomoslicing of transparent solids.

Light, science & applications·2026
Same journal

Multi-dimensional spatial-temporal projection ultrafast compressed imaging.

Light, science & applications·2026
Same journal

Expanded field of view light-field extended-reality displays with metalens array.

Light, science & applications·2026
Same journal

Experimental observation of counter-intuitive features of photonic bunching.

Light, science & applications·2026
Same journal

High-speed and high-sensitivity multi-gas detection based on parallel heterodyne LITES sensor.

Light, science & applications·2026
查看所有相关文章

相关实验视频

Updated: May 7, 2025

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research
05:22

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

Published on: June 21, 2024

280

用于高保真度解卷显微镜的多分辨率分析.

Baolei Liu1, Fan Wang2

  • 1School of Physics, Beihang University, 100191, Beijing, China. liubaolei@buaa.edu.cn.

Light, science & applications
|December 31, 2024
PubMed
概括
此摘要是机器生成的。

一个新的解卷算法提高了光显微镜的分辨率,并减少了噪声. 这一进步允许在研究中进行更精确,更详细的生物成像.

更多相关视频

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

9.6K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

9.8K

相关实验视频

Last Updated: May 7, 2025

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research
05:22

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

Published on: June 21, 2024

280
Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

9.6K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

9.8K

科学领域:

  • 显微镜的使用方法
  • 图像分析 图像分析
  • 生物技术是生物技术.

背景情况:

  • 光显微镜对于生物研究至关重要.
  • 分辨率和噪声的限制阻碍了详细的细胞成像.
  • 需要先进的解卷技术来克服这些局限性.

研究的目的:

  • 为光显微镜开发一种新的解卷算法.
  • 为了提高图像分辨率和改善噪音控制.
  • 为了实现更准确和详细的生物成像.

主要方法:

  • 开发了一个保证忠实性的多分辨率分析解卷算法.
  • 该算法应用于光显微镜数据集.
  • 基于提高分辨率和降低噪音来评估性能.

主要成果:

  • 该算法显著提高了图像分辨率.
  • 实现了有效的噪音控制.
  • 对生物样本的成像质量得到了提高.

结论:

  • 开发的算法为光显微镜提供了卓越的性能.
  • 它为先进的生物研究提供了有价值的工具,需要高度细节的成像.
  • 这种方法提高了显微镜数据的准确性和可靠性.