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Updated: May 7, 2025

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在三维生物成像中的光学切割方法

Jing Zhang1,2, Wei Qiao1,2, Rui Jin1,2

  • 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China.

Light, science & applications
|December 31, 2024
PubMed
概括

本文回顾了光学切割显微镜技术,以实现更清晰的3D生物成像. 它根据照明/检测轴对方法进行分类,并为选择特定成像需求的最佳技术提供指导.

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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