一种多重微阵列侧流免疫测试装置,用于同时确定大米中的五种真菌毒素
Saowalak Adunphatcharaphon1,2, Oluwatobi Kolawole1,3, Thanasat Sooksimuang1,4
1International Joint Research Center on Food Security (IJC-FOODSEC), Khlong Luang, Pathum Thani, 12120, Thailand.
NPJ science of food
|December 31, 2024
概括
一种新的环保方法可以快速地在现场检测大米中五种常见的真菌毒素. 这种创新的测定方法通过准确量化低于法规限制的有害污染物来确保食品安全.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 环境科学 环境科学
背景情况:
- 毒素在食品中的同时存在对人类和动物健康构成重大风险.
- 准确和快速的检测方法对于确保食品安全至关重要.
- 现有的真菌毒素检测方法可能耗时,需要专门的设备.
研究的目的:
- 开发一种环保的现场方法,同时检测大米中的五种受管制的真菌毒素.
- 创建一个创新的基于多重微阵列的侧流免疫试验.
- 使用一种新型的便携式阅读器快速量化菌毒素.
主要方法:
- 使用生物基溶剂开发一种环保,超快速的提取方法.
- 基于多重微阵列的侧流免疫试验的实施.
- 使用一种新的发光有机染料用于信号生成.
- 使用便携式阅读器,具有用户友好的接口来获取数据.
主要成果:
- 在大米中成功地同时确定了阿弗拉托辛B1,T-2毒素,泽拉伦,脱氧尼瓦伦和烟素B1.
- 实现的恢复率在77%至127%之间.
- 检测极限在0.56至1.89μg/kg之间,低于法规限制.
- 相对标准偏差始终低于25%.
结论:
- 开发的分析系统提供了一个准确和高效的现场方法,用于在米中检测多种真菌毒素.
- 环保的方法和便携式阅读器有助于快速评估食品安全.
- 这种方法解决了全球越来越关注食品中真菌毒素污染的问题.
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