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使用PacBio HiFi测序和Hi-C技术进行Triplophysa bombifrons的染色体级基因组组装

Chengxin Wang¹, Site Luo², Yong Song¹

¹College of Life Science and Technology/Tarim Research Center of Rare Fishes, Tarim University, CN-0997, Alar 843300, Xinjiang Uygur Autonomous Region, Xinjiang, China.

Scientific data

|December 31, 2024

概括

本研究介绍了使用先进的测序技术,对Triplophysa bombifrons鱼的第一个染色体水平基因组组. 高质量的基因组为了解鱼类遗传学和保护工作提供了宝贵的资源.

科学领域:

基因组学就是基因组学.
鱼类学鱼类学鱼类学
生物信息学是一种生物信息学.

背景情况:

中国特有鱼种Triplophysa bombifrons的遗传数据有限,这阻碍了研究.
现有的遗传信息主要局限于它的线粒体基因组,使核基因组在很大程度上未被探索.

研究的目的:

为了生成Triplophysa bombifrons的高质量的染色体级基因组组合.
为T. bombifrons提供一个基础的基因组资源,用于未来的保护研究,功能基因组学和进化研究.

主要方法:

利用PacBio长读测序和Hi-C染色体相互作用映射进行基因组组.
采用了新的,基于同质的和基于转录的基因预测方法.
通过使用脊椎动物基准测试 (BUSCO) 评估基因组完整性.

主要成果:

成功组装了655.95Mb的染色体级基因组,包括25个染色体.
实现了24.25 Mb的高支架N50和97.4%的BUSCO完整性.
鉴定并功能性注释了34,211个基因.

结论:

染色体水平的基因组组合代表了Triplophysa bombifrons研究的重大进展.
这种基因组资源将支持 Triplophysa 属内的保护策略,功能基因组学,分子育种和进化分析.

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.