在米改进过程中插入/删除变化的基因组模式
Xia Zhou1, Jilong Li2,3, Lei Chen4
1Urban Construction School, Beijing City University, Beijing, 101300, China.
BMC genomics
|December 31, 2024
概括
本研究确定了大米的插入/删除 (INDEL) 变异,揭示了它们的分布和与关键农学特征的关联. 这些发现为改善大米品种提供了宝贵的遗传资源.
科学领域:
- 植物遗传学 植物遗传学
- 提高作物质量 的方法.
- 生物信息学是一种生物信息学.
背景情况:
- 米是重要的主食作物,对全球粮食安全至关重要.
- 米的遗传改进依赖于了解其遗传变异.
- 虽然单核酸多态性 (SNP) 已得到充分研究,但大米遗传中的插入和删除 (INDEL) 仍未得到充分研究.
研究的目的:
- 分析改良大米品种的插入/删除 (INDEL) 变异模式.
- 为了确定与大米重要的农学特征相关的INDEL.
- 为米育种和改良提供遗传资源.
主要方法:
- 从148个大米品种的重新排序数据中提取INDEL.
- 全基因组关联研究 (GWAS) 整合INDEL和表型数据.
- 对候选基因的哈普洛型分析.
主要成果:
- 确定了938,585个INDEL,其中89.0%的长度为2-10 bp.
- INDEL分布不均,其中33个热点区域和47.0%位于基因附近.
- 发现了6331个与五个农学特征相关的显著位置,包括像OsMED25,OsRRMh和MOC2.2这样的基因.
结论:
- 在栽培大米中表征INDEL变异模式.
- 建立了INDEL和关键农业特征之间的关联.
- 产生了有价值的遗传见解和资源,用于未来的米改进.
更多相关视频
06:10Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
7.3K
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
12.2K
相关概念视频
Comparing Copy Number Variations and SNPs
16.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
16.7K
Plant Breeding and Biotechnology
18.5K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.5K
Single Nucleotide Polymorphisms-SNPs
13.5K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
13.5K
Overview of Transposition and Recombination
15.0K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.0K
Transgenic Plants
7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K
Gene Evolution - Fast or Slow?
7.0K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.0K
