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格子光片显微镜允许超高分辨率的成像受体在叶子组织中的受体

Jeremiah Traeger¹, Mengran Yang², Gary Stacey²

¹Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington.

Biophysical journal

|January 1, 2025

概括

格子光片显微镜克服了叶绿素背景光,使植物免疫受体的超分辨率成像成为可能. 这项技术可视化了在刺激后Arabidopsis thaliana叶细胞中的蛋白质内化和同位化变化.

科学领域:

植物细胞生物学植物细胞生物学
显微镜技术的使用方法
分子植物病理学分子植物病理学

背景情况:

植物叶子成像是具有挑战性的,因为叶绿素自光.
超分辨率显微镜需要克服背景噪声来进行详细的细胞分析.
了解植物免疫受体动态对于植物防御研究至关重要.

研究的目的:

应用超高分辨率显微镜与格子光板照明,用于成像Arabidopsis thaliana叶细胞.
在纳米尺度上可视化免疫受体蛋白的局部化和动态.
研究诱导因子对类似受体激酶内部化和局部化的影响.

主要方法:

使用晶格光片显微镜与STORM超分辨率相结合.
在Arabidopsis thaliana叶细胞中的局部免疫反应蛋白 (P2K1和FLS2).
重建了三维的蛋白质位置,并分析了局部化变化.

主要成果:

在植物叶子组织中实现了100nm以下的分辨率成像,克服了叶绿素背景.
观察到P2K1和FLS2受体在用ATP和flg22诱导剂刺激时显著内化.
经过刺激后表膜蛋白的局部化下降,表明了不同的内部化途径.

结论:

格子光板显微镜在具有挑战性的植物组织中有效用于超分辨率成像.
这项研究为植物免疫受体动力学和内部化机制提供了纳米级的见解.
研究结果表明,受体内部化在植物免疫信号通路中起着作用.

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