在冠状动脉疾病中识别内细胞网膜与压力相关的生物标志物
Yuanyuan Lin1,2, Lin Ni2, Luqun Yang2
1Department of Nuclear Medicine First Hospital of Shanxi Medical University Shanxi Medical University, Taiyuan, Shanxi 030001, China.
这项研究确定了八个与内质网膜压力 (ERS) 相关的基因,作为冠状动脉疾病 (CAD) 的新生物标志物. 这些生物标志物显示出诊断潜力,并为CAD提供新的治疗点.
科学领域:
- 心血管生物学 心血管生物学
- 分子医学是分子医学.
- 基因组学就是基因组学.
背景情况:
- 冠状动脉疾病 (CAD) 的发病包括动脉样硬化病变.
- 细胞内膜网膜应激 (ERS) 在CAD中的特定作用仍然不清楚.
研究的目的:
- 识别和验证与ERS相关的基因作为CAD的潜在生物标志物.
- 探索这些生物标志物的诊断和预测价值.
主要方法:
- 在CAD中差异表达ERS相关基因的生物信息分析.
- 最小绝对收缩和选择操作员 (LASSO) 回归用于生物标志物选择.
- 接收器操作特征 (ROC) 分析,人工神经网络 (ANN) 和名ogram建模用于验证.
- 定量逆转录-聚合酶连锁反应 (qRT-PCR) 用于实验验证.
主要成果:
- 在CAD中鉴定出13个差异表达的ERS相关基因 (DEERSRGs),富含ER通路.
- 通过LASSO和ROC分析验证了八个基因 (RCN2,HRC,DERL2,RNF183,CRH,TMED2,PPPP1R15A,IL1A) 作为强大的CAD生物标志物.
- 生物标志物显示出显著的诊断和预测能力,与免疫细胞透和免疫调节器相关联.
- qRT-PCR证实了关键生物标志物的表达模式,与生物信息学发现保持一致.
结论:
- 八个与ERS相关的基因作为CAD诊断和预后的可靠生物标志物.
- 这些生物标志物与免疫反应有关,这表明潜在的治疗含义.
- 这些发现为CAD中的ERS机制以及潜在的治疗策略提供了新的见解.
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