乳杆菌通过调节RFTN1/脂质/TLR4-介导炎症来保护慢性辅助性中耳炎
Zhuohui Liu1, Fan Zhang1, Fengfeng Jia1
1Department of Otolaryngology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.
Biologics : targets & therapy
|January 1, 2025
概括
乳酸菌在治疗慢性性中耳炎 (CSOM) 中具有潜力. 这种益生菌疗法通过抑制RFTN1-脂质-TLR4通路,减少炎症和预防听力损失而起作用.
科学领域:
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 慢性性中耳炎 (CSOM) 是全球可预防的听力损失的主要原因.
- 益生菌疗法,特别是乳酸菌,正在针对传染性和炎症性疾病进行探索,但它们在CSOM中的作用尚不清楚.
研究的目的:
- 调查乳酸菌对CSOM的抗病原作用.
- 阐明Lactobacillus在CSOM中的作用的基础分子机制.
主要方法:
- 从CSOM患者和对照组中中耳颗粒的RNA测序.
- 在人类中耳上皮细胞和老鼠中使用Bacillus cereus和Staphylococcus aureus构建CSOM模型.
- 西方斑块,qPCR,脂质标签,ELISA和HE染色以分析RFTN1,脂质,TLR4和炎症反应.
- 在CSOM大鼠模型中评估Lactobacillus的治疗效果.
主要成果:
- RNA测序确定了3646个在CSOM中差异表达的基因,具有高RFTN1表达.
- 抑制RFTN1降低了CSOM炎症反应和脂质形成,并降低了脂质内的TLR4表达.
- 乳酸菌治疗通过减少RFTN1和TLR4表达来减轻CSOM的进展,这表明潜在的抗炎作用.
结论:
- 乳酸菌在缓解CSOM进展方面发挥着重要作用.
- 这项研究揭示了一种涉及乳杆菌调节的抑制RFTN1-脂质-TLR4信号通路在CSOM中的机制.
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