猪G9P[23]和G5P[23]在小猪中的RVA之间的致病性比较
Zixin Li1, Yudi Pan1, Yanxiang Zhou1
1State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, PR China.
Veterinary microbiology
|January 1, 2025
概括
新兴的G9轮状病毒 (RVA) 菌株,如923H,在小猪中表现出更高的毒性和感染力,而不是以前占主导地位的G5 RVA菌株 (NG523). 这项研究解释了在猪群中G9 RVA的患病率的增加.
科学领域:
- 兽医病毒学 兽医病毒学
- 动物病理学 动物病理学
- 分子流行病学分子流行病学
背景情况:
- 罗塔病毒A组 (RVA) 在牲畜中引起显著的病毒性腹,导致经济损失.
- 猪轮状病毒 (PoRV) 是养猪业的一个主要问题,G5在历史上占主导地位.
- G9 RVA是一种新兴的基因型,在人类和动物种群中越来越普遍.
研究的目的:
- 为了比较新出现的G9P[23] (923 H) 和流行的G5P[23] (NG523) 猪轮状病毒菌株的体外和体内特征.
- 调查导致猪中G9 RVA患病率上升的病原机制.
主要方法:
- 猪轮状病毒G9P[23] (923 H) 和G5P[23] (NG523) 的分离和表征.
- 使用猪小肠上皮细胞进行体外生长曲线分析.
- 在体内对小猪的毒性评估,包括病毒载荷的确定和肠道和肠外组织的组织病理学检查.
主要成果:
- 与G5P[23]菌株 (NG523) 相比,G9P[23]菌株 (923 H) 在猪小肠上皮细胞中表现出更高的敏感性.
- 在体内,923H表现出更强的毒性,在小猪中引起更严重的临床症状和病理损伤.
- 在感染小猪的肠道和肠外组织中检测到G9 RVA的较高病毒载荷,并观察到显著的肺病理.
结论:
- 新出现的G9P[23]轮状病毒菌株 (923 H) 比小猪的G5P[23]菌株 (NG523) 更具有病原性和毒性.
- 增加的致病性和病毒传播有助于猪群中G9 RVA的日益流行.
- 这些发现为推动G9 RVA在养猪场广泛流行的机制提供了关键的见解.
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