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类似雌激素的化合物严重扰乱了日本长中生殖细胞的形成

Yuya Ogawa¹, Shusei Mizushima¹, Asato Kuroiwa¹

¹Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan.

Biochemical and biophysical research communications

|January 1, 2025

概括

雌激素内分泌干扰化学物质 (EDC) 干扰鸟类生殖细胞的发育,影响男性和女性的生育能力. 新的蛋白质标记物识别了这些影响,揭示了生殖细胞的损伤和分化受损.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
发展生物学发展生物学

背景情况:

鸟类生殖细胞分化成繁荣生殖和 oogonia 对于生育至关重要.
雌激素内分泌干扰化学物质 (EDC) 被怀疑会干扰鸟类生殖细胞的发育.
缺乏特定的分子标记物阻碍了以前关于EDC对鸟类生殖系分化的影响的研究.

研究的目的:

为了确定可靠的分子标记物,鸟类的繁荣matogonia和 oogonia.
研究特定雌激素EDCs对鸟类生殖细胞形成和分化在卵中的影响.
评估EDCs对日本的雄性和雌性生育能力的影响.

主要方法:

配对RNA测序和液体染色学质谱测量,以识别生殖细胞标记蛋白.
在日本的卵胚胎体内暴露于二甲基stilbestrol (DES),o,p'-DDT,乙inylestradiol (EE) 和双A.
对生殖细胞的增殖,分化 (ITGB1,IGF2BP1,STRA8表达) 和生殖腺发育的分析.

主要成果:

综合素β1 (ITGB1),IGF2BP1和STRA8被确定为繁荣性和 oogonia 的可靠标志物.
德斯通过抑制增殖和分化成ITGB1阳性细胞,严重损害了男性生殖细胞.
在男性中,DES和o,p'-DDT减少了女性生殖细胞数量,而EE则导致IGF2BP1生殖细胞减少和部分卵巢分化.
所有测试的EDC都导致卵巢分化受损,导致STRA8阳性 oogonia.

结论:

雌激素EDCs破坏鸟类生殖细胞的发育,导致男性和女性的生育能力下降.
已识别的分子标记物 (ITGB1,IGF2BP1,STRA8) 允许精确评估EDC诱导的生殖细胞毒性.
在鸟类化期间,雌激素EDCs的生物积累对生殖健康构成重大威胁.

关键词:

这是异常的异常.雌激素的内分泌干扰化学物质.胚胎细胞的形成乌戈尼乌尼奥姆 (Oogonium) 是一种有机物.原始的生殖细胞.精子子 (Spermatogonium) 是一种具有精子的物质.

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