相关实验视频

Updated: Jun 4, 2025

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

一个空间分辨的多组单细胞大豆发展图谱

Xuan Zhang¹, Ziliang Luo¹, Alexandre P Marand²

¹Department of Genetics, University of Georgia, Athens, GA, USA.

|January 1, 2025

概括

这项研究绘制了不同细胞类型的大豆基因调节图,揭示了控制基因表达的特定 cis 调节元素. 它为了解大豆发育和固提供了基础资源.

科学领域:

植物生物学
基因组学
分子生物学

背景情况:

对控制基因表达至关重要.
了解大豆中的CREs对于改善作物至关重要.

研究的目的:

创建一个关于大豆基因表达和染色质可访问性的综合图谱.
识别细胞类型特定的CREs和转录因子 (TF) 动机.
研究大豆发育中的基因调控网络和共生固.

主要方法:

在十种大豆组织中生成具有空间分辨率的基因表达和染色质可访问性的单细胞地图.
识别不同的细胞类型和可访问的染色体区域 (ACR).
在监管网络中分析TF动机的丰富和保存.

主要成果:

确定了103种不同的大豆细胞类型和303,199个ACR.
发现近40%的ACR表现出特定细胞类型的模式.
发现了细胞类型特异性TF动机,发育轨迹以及DOF11和homeoboxTF等关键调节物.

结论:

这项研究为分析大豆的基因调控程序提供了宝贵的资源.
确定了内和胚胎发育的关键调节剂,包括糖运输体和同源体TF.
对豆类中固基因调节网络的保护提供了洞察力.

关键词:

其他国家其他监管要素胚胎发生基因调控网络多种类型单细胞地图大豆的发展空间转录学同生固定

更多相关视频

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

相关概念视频

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.