基于体外多重毒性测试对双A及其类似物安全性的全面评估
Juan Wang1, Xiangsheng Hong2, Wang Liu3
1National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; National Joint Research Center for Ecological Conservation and High Quality Development of the Yellow River Basin, Beijing 100012, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Journal of hazardous materials
|January 1, 2025
概括
双相似物 (BPs) 通常显示出比双A (BPA) 更大的毒性. 许多BP破坏内分泌功能并增加细胞亡,表明需要进一步调查的潜在健康风险.
科学领域:
- 环境毒理学环境毒理学
- 内分泌学 在内分泌学.
- 细胞毒理学 细胞毒理学
背景情况:
- 双A (BPA) 替代品,称为双类型 (BPs),在环境中经常被检测到.
- 防腐剂的安全性概况尚未确立,这引发了公众健康方面的担忧.
研究的目的:
- 评估九种BP和BPA的细胞毒性,内分泌干扰,神经毒性,酸碳化合物受体 (AhR) 活性和基因毒性.
- 使用ToxPi评分系统,比较BP与BPA的综合毒性.
主要方法:
- 在三个细胞系上进行了细胞毒性测试,以确定IC50值.
- 通过评估雌激素和抗雄激素效应来评估内分泌干扰.
- 通过测量SH-SY5Y细胞中的亡来评估神经毒性.
- 通过测量7-埃托克西雷索鲁芬-O-脱乙酶水平来确定AhR活性.
- 基因毒性通过量化DNA损伤来评估.
- 使用ToxPi评分系统来对BP的整体毒性进行排名.
主要成果:
- 超过一半的测试BP显示细胞毒性比BPA更高,IC50和LogKow之间的线性相关性.
- 所有BP都表现出内分泌干扰作用,其中BPAF和BPAP显示出广泛的内分泌抑制影响.
- 在SH-SY5Y细胞中的BP (BPP除外) 和BPA诱导的亡在1-10μM.
- BPF和BPP显著激活了AhR,而BPAF在比BPA,BPF和BPP更低的度下诱导了DNA损伤.
- 在9个BP中,有7个获得了比BPA更高的ToxPi得分,这表明了更大的综合性毒性.
结论:
- 双相似物通常比BPA具有更强的综合毒性.
- 这些发现突显出迫切需要进一步研究BP对健康的潜在影响.
- 由于其广泛的检测和毒性,BP引发了严重的环境和健康问题.
相关概念视频
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Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
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Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
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