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从Streptococcus suis血清型2中鉴定的一种表面蛋白通过其多糖囊表现出对中性粒细胞的抗性能力

Fang Ma¹, Guangyu Wang², Zhe Ma³

¹MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China; Institute of Veterinary Immunology & Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.

Microbial pathogenesis

|January 1, 2025

概括

葡萄球菌 suis 血清型 2 避开免疫细胞使用其囊. 移除细胞表面蛋白 (CSP) 暴露了囊,增加了免疫细胞的攻击,减少了细菌的存活率,揭示了控制猪 estreptococcosis 的潜在目标.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
动物传播疾病动物传播疾病

背景情况:

斯特雷波氏菌 suis 血清型 2 (SS2) 是一种新兴的动物感染性病原体,引起各种疾病.
SS2败血症表明先天免疫细胞防御的逃避,但机制尚不清楚.
中性粒细胞对抗细菌感染的天生的免疫力至关重要.

研究的目的:

研究SS2逃避中性粒细胞介导免疫的机制.
确定参与SS2生存的细菌毒性因子,以对抗先天免疫细胞.
探索预防和控制猪链球菌病的潜在目标.

主要方法:

一个细胞表面蛋白 (CSP) 删除突变体 (ΔCSP) 的SS2.2的构建和特征.
评估中性粒细胞外细胞陷 (NET) 的释放和反应性氧物种 (ROS) 的形成.
在中性粒细胞和NET中评估细菌的生存和繁殖.
对细胞外信号调节激酶1/2 (ERK1/2) 在中性粒细胞中发出信号的分析.

主要成果:

突变的ΔCSP显示出一个有缺陷的多糖囊和增强的生物膜形成.
与野生类型SS2相比,ΔCSP诱导了NET释放和ROS产量的增加.
细菌的生存和增殖在中性粒细胞和DCSP的NET中显著降低.
纯化的SS2多糖囊抑制了中性粒细胞的杀菌活性.
ΔCSP刺激了中性粒细胞中的ERK1/2信号,可能增强了杀死细菌的作用.

结论:

该SS2多糖囊是抑制中性粒细胞清除的关键毒性因子.
细胞表面蛋白 (CSP) 在SS2逃避天生的免疫力中起作用.
准SS2囊或CSP可能是控制猪菌株菌根的策略.

关键词:

宿主-病原体相互作用它们对中性粒细胞具有抗性.多糖囊中的多糖.链球菌 suis 血清型 2 类型病毒性因素是病毒性因素.

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