[获得初级骨髓细胞和建立初级骨髓细胞的B-ALL小鼠模型]
Li Zhao1,2, Dong-Hai Tang1,2, Chun-Xiao Ren1,2
1Blood Disease Institute, Xuzhou Medical University,Xuzhou 221000, Jiangsu Province, China.
Zhongguo shi yan xue ye xue za zhi
|January 1, 2025
概括
研究人员通过在体内采集和传递初级Ph + 细胞成功建立了费城染色体阳性B型急性淋巴细胞白血病 (B-ALL) 的小鼠模型. 这一突破使得进一步研究B-ALL进展和潜在的治疗方法成为可能.
科学领域:
- 血液学 血液学 血液学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 乙级急性淋巴细胞白血病 (B-ALL) 是一种严重的血液性恶性瘤.
- 费城染色体 (Ph) 是某些B-ALL病例的标志性遗传变异.
- 建立可靠的临床前模型对于理解B-ALL病变发生和开发向治疗至关重要.
研究的目的:
- 从B-ALL.中分离和传播主要的费城染色体阳性 (Ph+) 细胞.
- 通过体内传递开发一个功能性小鼠模型来检测Ph+ B-ALL.
- 描述已建立的B-ALL小鼠模型的免疫类型和病理特征.
主要方法:
- BCR-ABL P210融合基因的逆转录病毒转移到C57BL/6J小鼠骨髓 (BM) 细胞中.
- 通过将转染的BM细胞注射到被辐射的同源性小鼠中,生成第一代 (G1) B-ALL模型.
- 在体内顺序传递Ph+细胞以建立后续代 (G2,G3,G4) 和B-ALL小鼠模型.
- 使用流细胞计,H&E染色和外周血液涂抹进行分析,以评估免疫表型和病理学.
主要成果:
- 输注的小鼠出现了B-ALL症状,包括体重减轻和.
- 周围血液涂抹显示了不成熟的淋巴细胞,H&E染色显示了白血病细胞透到器官中.
- 流细胞计证实了CD19+NGFR+Ph+B淋巴细胞与序列传递的逐渐增加 (G1中的19.0%,G2中的47.3%,G3中的61.0%).
- 免疫类型分析表明稳定的移植和Ph + B淋巴细胞在通道上的纯度增加.
结论:
- 该研究成功地在体内获得并通过了初级Ph+细胞.
- 有效地建立了费城染色体阳性B型急性淋巴细胞白血病 (B-ALL) 的强大的小鼠模型.
- 开发的模型为未来的B-ALL研究提供了一个有价值的平台.
相关概念视频
Preparation of 1° Amines: Gabriel Synthesis
3.4K
Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
3.4K
Acute Pharyngitis
188
Introduction
Acute pharyngitis is the inflammation of the back of the throat (pharynx), commonly resulting in a sore throat. It is a frequently encountered condition that prompts individuals to seek medical advice.
Classification
Acute pharyngitis can be categorized based on its underlying cause:
Acute pharyngitis is the inflammation of the back of the throat (pharynx), commonly resulting in a sore throat. It is a frequently encountered condition that prompts individuals to seek medical advice.
Classification
Acute pharyngitis can be categorized based on its underlying cause:
188
Chronic Pharyngitis
505
Chronic pharyngitis refers to persistent inflammation of the pharyngial mucosa.
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
505
Preparation of 1° Amines: Hofmann and Curtius Rearrangement Overview
3.1K
In the presence of an aqueous base and a halogen, primary amides can lose the carbonyl (as carbon dioxide) and undergo rearrangement to form primary amines. This reaction, called the Hofmann rearrangement, can produce primary amines (aryl and alkyl) in high yields without contamination by secondary and tertiary amines.
3.1K
Preclinical Development: Overview
4.0K
Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...
4.0K
Direct-Acting Cholinergic Agonists: Pharmacokinetics
973
Direct-acting cholinergic agonists, such as synthetic choline esters and naturally occurring alkaloids, exert their effects by enhancing the actions of acetylcholine and stimulating the parasympathetic nervous system. Synthetic choline esters share structural similarities with acetylcholine. For example, they have a positively charged quaternary ammonium or onium group, contributing to their hydrophilic characteristics. As a result, they are poorly absorbed in the body through oral...
973


