相关实验视频

Updated: Jul 6, 2026

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

Published on: February 12, 2015

在3D组织工程模型中,电子烟对口腔微生物组和新陈代谢的影响

Meenu Maan¹, Jisha Pillai U², Dalia Alsadig Mohamed³

¹Department of Restorative Dentistry, Hamdan Bin Mohammed College of Dental Medicine (HBMCDM), Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU), Dubai Health, Dubai, United Arab Emirates; Department of Biophysics, New York University-Abu Dhabi, Abu Dhabi, United Arab Emirates.

International dental journal

|January 1, 2025

概括

电子烟 (EC) 使用显著改变了口腔微生物组和代谢组. 在3D口腔粘膜模型中,含尼古丁的风味EC气溶暴露显示出不良影响,影响与口腔癌相关的途径.

科学领域:

口腔健康研究口腔健康研究
微生物组和代谢组学
在体外建模模型.

背景情况:

电子烟 (EC) 的使用破坏了口腔微生物组的组成和多样性.
已知由于使用EC而导致粘膜细胞中的代谢途径受损.
对EC暴露对口服代谢组的影响仍未得到充分研究.

研究的目的:

调查EC气溶暴露在口腔微生物组和代谢组失调中的作用.
用人口腔粘膜的体外3D器官模型进行模拟.
评估风味EC气溶,有或没有尼古丁的效果.

主要方法:

生成的3D组织工程人类口腔粘膜模型.
从健康的捐赠者唾液中感染口腔微生物的模型.
使用模拟设备,暴露在EC气溶 (调味品,含尼古丁或不含尼古丁) 的模型.
通过16S rRNA基因测序分析微生物组组成.
进行气相色谱-质谱测量以进行代谢组概况.

主要成果:

与对照组相比,在含尼古丁组的调味EC中观察到更多的α多样性.
在对照组和EC暴露组之间确定了不同的代谢概况.
在含有尼古丁的调味EC组中,13种代谢物显著增加,表明了不良影响.
改变的代谢物与与口腔癌进展相关的途径有关.

结论:

使用EC显著影响口腔健康.
证实了口腔微生物组和代谢物概况的变化.
一个临床相关的体外3D模型证明了EC气溶暴露的不良影响.

关键词:

电子烟是一种电子烟.代谢组的个人资料.微生物组是一个微生物组.在口腔的粘膜.组织工程是组织工程.

更多相关视频

Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System

Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System

Published on: May 24, 2017

Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies

Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies

Published on: August 25, 2018

相关概念视频

The Oral Microbiota

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...