Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
LTR Retrotransposons03:08

LTR Retrotransposons

17.3K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.3K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.2K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.2K
DNA-only Transposons02:57

DNA-only Transposons

14.3K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.3K
  1. 首页
  3. 通过arabidopsis的cenh3染色体进行中心性逆转移素的集成
  1. 首页
  3. 通过arabidopsis的cenh3染色体进行中心性逆转移素的集成

相关实验视频

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

3.8K

通过Arabidopsis的CENH3染色体进行中心性逆转移素的集成

Sayuri Tsukahara1, Alexandros Bousios2, Estela Perez-Roman3

  • 1Department of Biological Sciences, The University of Tokyo, Tokyo, Japan. stsukaha@g.ecc.u-tokyo.ac.jp.

Nature
|January 1, 2025

在PubMed 上查看摘要

概括
此摘要是机器生成的。

中转基因进化涉及快速变化的重复序列和逆转基因. 这项研究揭示了特定的逆转移子如何向中转基因,推动植物和其他真核生物的快速中转基因进化.

更多相关视频

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

9.5K
Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
12:36

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

20.4K

相关实验视频

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

3.8K
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

9.5K
Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
12:36

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

20.4K

科学领域:

  • 遗传学
  • 分子生物学
  • 进化生物学

背景情况:

  • 对于染色体分离至关重要的中间体,其特点是快速演变的重复序列,如合重复 (TR) 和可转移元素 (TE).
  • 中核特异性基因素H3 (CENH3) 是一个关键成分,其相关区域通常包含这些动态重复序列.
  • 通过反转移体,特别是"心爱体"的机制,准和影响中心体进化是不清楚的.

研究的目的:

  • 通过阿拉比多普西斯的逆转移体对中心体的向机制进行研究.
  • 了解逆转移子的整合和转换如何促进快速的中心体进化.
  • 识别决定它们的染色体向特异性的逆转移体的结构变异.

主要方法:

  • 在Arabidopsis物种中分析逆转移素动态 (Ty3和Ty1).
  • 将Ty1/Copia元素Tal1纳入Arabidopsis thaliana中CENH3占用区域的新整合研究.
  • 检查子宫外CENH3区域扩张和Tal1整合模式.
  • 嵌合式TEs的整合光谱分析,以确定染色体向的结构决定因素.

主要成果:

  • Ty3和Ty1长端反复转移体在阿拉比多普西斯的中心转移体中呈现出快速的循环.
  • Tal1逆转移子重新集成到Arabidopsis thaliana中的CENH3结合区域.
  • CENH3区域的扩张导致了Tal1整合场所的扩张.
  • 基因 TE 分析发现了结构变异,使中心基因与富基因染色体具有差异性向特异性.

    • 结论:

    • 中心色素成分显著影响TE的整合和循环,推动了快速的中心色素演变.
    • 受结构变异影响的逆转移子向机制在形成细胞中心结构和真核生物的进化中起着至关重要的作用.
    • 这些发现为基因组进化中的TEs和中心色素之间的相互作用提供了洞察力.