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在波兰野生动物中通过分子和血清学技术识别Toxoplasma gondii

Joanna Nowicka¹, Daniela Antolova², Anna Lass¹

¹Medical University of Gdańsk, Poland.

Annals of agricultural and environmental medicine : AAEM

|January 2, 2025

概括

野生动物作为Toxoplasma gondii的水库,有助于其在环境中的存在. 建议使用ELISA测试来对小哺乳动物中的这种寄生虫进行生物监测,其性能优于分子方法.

科学领域:

兽医寄生虫学兽医寄生虫学
野生动物流行病学
环境健康环境健康

背景情况:

动物是已知的*Toxoplasma gondii*寄生虫的储存库,这种寄生虫对公共卫生有重大影响.
了解野生动物在维持T. gondii中的作用对于环境监测至关重要.
需要有效的生物监测策略来跟踪野生动物种群中的寄生虫流行情况.

研究的目的:

评估野性动物在维持 *Toxoplasma gondii* 的作用.
分析7种野生动物物种中T. gondii的流行率和血清流行率.
为了比较小哺乳动物T. gondii生物监测的分子和血清学方法的有效性.

主要方法:

从波兰东北部收集的动物样本.
使用PCR和嵌套PCR测定对大脑,脏和血液样本进行分子分析.
在血清样本上使用ELISA和凝结试验进行血清分析.

主要成果:

在使用PCR检测的68只动物样本中,没有检测到*Toxoplasma gondii*DNA.
凝结试验没有产生任何阳性结果.
对T. gondii的抗体在56个分析血清中的5个中被检测到 (血清流行率=8.9%).

结论:

据证实,动物参与了T. gondii的生命周期,作为草地水库.
与分子方法相比,ELISA测试在检测T. gondii*抗体方面表现出更高的有效性.
建议ELISA用于未来对*T. gondii*在小型哺乳动物种群中的生物监测.

关键词:

这种毒性淋巴细胞 (Toxoplasma gondii) 是生物监测生物监测环境污染污染环境污染通过动物传播的疾病.动物动物动物野生动物野生动物

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