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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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相关实验视频

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Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
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一个圆顶的窗户室用于多模式的光学成像.

Photini Rice1, Makenna Aitken1, Hasina Shir1

  • 1Biomedical Engineering, The University of Arizona, Tucson, AZ, USA.

BioTechniques
|January 2, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新型的背部皮肤窗室,其中有一个圆顶的化乙烯 (FEP) 窗户. 这种修改使多模体体内成像使用光板光显微镜 (LSFM),光学相干断层扫描 (OCT) 和多光子显微镜 (MPM).

关键词:
背部皮肤上的皮.化乙烯烯的化乙烯.光片光显微镜 光显微镜光片成像光片成像光片成像多光子显微镜的使用方法光学连贯性断层扫描技术

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科学领域:

  • 生物医学工程 生物医学工程
  • 显微镜的使用方法
  • 光学成像技术的成像

背景情况:

  • 背部皮肤片的窗户室对于体内显微镜来说至关重要.
  • 目前带有平面玻璃窗的室内限制了光板光显微镜 (LSFM) 的性能.
  • 为了获得最佳的成像,LSFM需要特定的样本几何和折射率匹配.

研究的目的:

  • 为增强多式模式体内成像设计一个修改后的窗口室.
  • 为了适应背部皮肤窗室用于光板光显微镜 (LSFM).
  • 为了实现同时进行光学相干断层扫描 (OCT),多光子显微镜 (MPM) 和LSFM.

主要方法:

  • 设计了一个修改后的窗户室,有一个由化乙烯烯 (FEP) 制成的圆顶观景窗.
  • 选择FEP材料是因为其折射率 (n) 与水和组织相似的性能相匹配.
  • 通过体外成像对原凝和微球幻影进行验证了室内设计.
  • 在自定义的鼠标平台上使用OCT,MPM和LSFM进行体内多模式成像.

主要成果:

  • 圆顶FEP窗口在体外显示对信号强度和图像分辨率的影响最小.
  • 修改后的腔室成功地满足了对样品定位和折射率的LSFM要求.
  • 在体内成功证明了同时进行OCT,MPM和LSFM成像.

结论:

  • 修改后的背部皮肤窗室与圆顶的FEP窗户兼容LSFM,OCT和MPM.
  • 这项创新扩大了临床前研究中的体内多式成像能力.
  • 设计方便研究生物过程的先进光学显微镜技术.