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在诱导的角外极化和差异化犬肠器官中,细菌的附着和结节运输功能

Shino Yoshida¹, Meg Nakazawa¹, Minae Kawasaki¹

¹Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, United States.

Frontiers in veterinary science

|January 2, 2025

概括

研究人员开发了一种新的狗肠道模型,使用角外结肠体. 这种模型增强了对狗的肠道疾病和宿主-病原体相互作用的研究,有利于犬类和人类翻译研究.

科学领域:

胃肠道学和兽医医学
翻译研究是翻译研究.
在体外建模模型.

背景情况:

狗与人类有类似的肠道疾病和肠道菌群,使它们成为有价值的大型动物模型.
传统的犬类器官模型面临着光线访问的挑战,阻碍了功能研究.
开发可访问的狗肠道模型对于推进胃肠道研究至关重要.

研究的目的:

为了研究狗的顶端外结肠器官 (colonoids) 中的极性逆转和差异化.
为了评估这些修改后的结肠杆菌的屏障完整性.
为了可视化宿主-病原体相互作用在狗结肠形模型.

主要方法:

诱导极性逆转和差异化在狗的顶部外结直肠.
评估结肠膜屏障的完整性.
使用致病性和非致病性大肠杆菌 (E. coli) 的宿主病原体相互作用的可视化.
对干细胞 (LGR5) 和肠上皮细胞 (ALPI,MUC2) 标记物的分析.

主要成果:

在保持屏障完整性的同时,成功实现了极性逆转和差异化.
极性逆转促进了对宿主-病原体相互作用的增强可视化.
对LGR5的下调和对ALPI的上调表明了区分.
通过增加ALPI和MUC2表达,进一步的差异化得到证实.

结论:

角外的狗结肠体代表了研究狗肠道疾病的生理学上相关的模型.
这种模型改善了对宿主-病原体相互作用和屏障功能的观察.
狗的结肠形状模型有可能促进狗和人类胃肠道的研究和治疗开发.

关键词:

顶端的-外的-外的狗类犬类动物狗类犬类不同化的差异化差异化.主体-病原体相互作用我们的肠道是肠道.这是一种有机物质的有机物质.极性逆转是指极性的逆转.

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