空腔H+-ATPase和美加林介导的前林素摄取:专注于超出 (Pro) Renin受体的元素
Na Wang1,2, Xifeng Lu2, A H Jan Danser1
1Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands.
Journal of cellular physiology
|January 2, 2025
概括
空腔H+-ATPase (V-ATPase) 亚单元影响质蛋白中介的质蛋白在细胞中的吸收. 向特定的V-ATPase元素可能为脏疾病提供新的治疗方法,并调节脏氨酸 - 血管新生素系统.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 梅加林是一种对蛋白质再吸收至关重要的受体.
- 梅加林作为前列宁的内细胞受体,依赖于前列宁受体 (PRR).
- PRR是真空H+-ATPase (V-ATPase) 复合体的一个辅助蛋白.
研究的目的:
- 为了研究单个V-ATPase子单元对大林介导的林蛋白吸收的影响.
- 探索参与这个过程的分子机制,包括适应蛋白和调节因素.
- 确定脏疾病的潜在治疗标和氨酸 - 血管新生素系统调节.
主要方法:
- 利用RNA干扰 (RNAi) 在表达巨素的BN16细胞中使单个V-ATPase亚单元沉默.
- 量化的大林的表达和林的吸收.
- 评估了适应蛋白 (ARH,Dab2),GSK3β酸化和内分泌网膜应激因子 (ERSF) 的水平.
主要成果:
- 沉默Atp6v0a1降低了19%的前林素吸收和表面大林素密度,与增加的GSK3β酸化有关.
- 抑制辅助蛋白Atp6ap1增加了15%的前林素吸收,增强了大林素的表达,并提高了ARH和ERSF的调节.
- 其他V-ATPase子单元的影响很小或没有显著影响.
结论:
- V-ATPase子单元通过差异调节大胆介导的林再吸收.
- 特定的V-ATPase成分代表了用于管理脏宁-血管素系统活性的新药学标.
- 这些发现为脏疾病提供了潜在的治疗策略,包括蛋白质再吸收受损,如丹特病.
更多相关视频
10:39Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Published on: June 12, 2013
13.5K
07:38Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
Published on: March 30, 2015
9.2K
相关概念视频
ATP Driven Pumps III: V-type Pumps
3.5K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.5K
Pinocytosis
3.1K
Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
Pinocytosis ("cellular drinking") is one of three main types of...
Pinocytosis ("cellular drinking") is one of three main types of...
3.1K
The Apoplast and Symplast
49.4K
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
49.4K
ATP Driven Pumps I: An Overview
7.7K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
7.7K
Intralumenal Vesicles and Multivesicular Bodies
3.3K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.3K
Transcellular Transport of Solutes
3.3K
Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
3.3K
