相关实验视频
Updated: Jun 4, 2025
09:13
Generation of Organoids from Mouse Extrahepatic Bile Ducts
Published on: April 23, 2019
9.8K
对胆囊和肝外胆道的形成,Sox17的积极自我调节是必要的
Linh T Trinh1,2,3, Ryan R Finnel1,2,3, Anna B Osipovich1,4
1Center for Stem Cell Biology , Vanderbilt University, Nashville, TN 37232, USA.
Development (Cambridge, England)
|January 2, 2025
概括
Sox17 (SRY-box转录因子17) 对于胆囊和胆道的发展至关重要. 即使Sox17水平的小幅降低也会破坏这种关键器官系统的形成.
科学领域:
- 发育生物学是发展生物学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 肝胰胆系统的形成需要SRY-box转录因子17 (Sox17) 的表达.
- 胆囊 (GB) 和肝外胆道 (EHBD) 的发育对Sox17水平敏感.
研究的目的:
- 研究Sox17表达水平对GB和EBHD发展的影响.
- 在开发过程中确定控制Sox17表达的cis-regulatory元素.
主要方法:
- 在小鼠Sox17基因中对促进子-近位突变的一系列等位基因的分析.
- 检查Sox17 TSS2促进体内的cis调节元件功能.
主要成果:
- 在TSS2促进体中删除SOX17结合的cis-regulatory元素会损害GB和EHBD的发展.
- 低于关键值的Sox17表达水平会破坏胆汁芽的生长.
- 一个依赖SOX17的自我调节循环维持了基本的Sox17表达水平.
结论:
- Sox17自我调节对于建立足够的度来促进GB和EBHD的发展至关重要.
- 在Sox17基因表达中发生的轻微干扰会显著影响新生的GB和EBHD系统的发展.
相关概念视频
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:24Liver Regeneration
3.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K