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相关概念视频

Mechanisms of Membrane-bending01:15

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The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
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The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most  widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
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活细胞膜表面的EMT诱导的形态变异

Hiroki Ida1,2,3,4,5, Noriko Taira5, Yuji Nashimoto2,6

  • 1Department of Electrical Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi 464-8601, Japan.

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概括

扫描离子导电显微镜 (SICM) 显示,上皮层-介质细胞转换 (EMT) 减少了细胞表面的纹. 通过监测这些纳米级膜变化,SICM表面测量可以跟踪EMT的进展.

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科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 显微镜的使用方法

背景情况:

  • 表皮细胞-介质细胞过渡 (EMT) 是一种关键的细胞过程,在这种过程中表皮细胞获得介质细胞表型.
  • 了解EMT诱导的细胞变化对于发育生物学和疾病研究至关重要.
  • 需要使用非侵入性的方法来观察纳米级的EMT.

研究的目的:

  • 在EMT期间使用扫描离子导电显微镜 (SICM) 调查纳米级膜变化.
  • 确定SICM是否可以作为评估EMT进展的指标.

主要方法:

  • 利用扫描离子导电显微镜 (SICM) 来进行对活细胞的纳米尺度可视化.
  • 进行时间间隔成像,观察EMT诱导期间的动态变化.

主要成果:

  • 随着EMT的进展,细胞表面的数量和大小下降.
  • 总体细胞形状的变化先于纳米尺度的形态变化.
  • 即使在EMT诱导后,小也表现出积极的运动.

结论:

  • 通过SICM,可以对EMT诱导的膜变化进行纳米级的非侵入性观察.
  • 细胞表面纹减少及其动态是EMT进展的潜在生物标志物.
  • 基于SICM的表面形状测量为评估EMT提供了有价值的工具.