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形态遗传性青素结合蛋白控制沙门氏菌中与毒性相关的III型分泌系统

Sónia Castanheira¹, Sara Torronteras¹, Juan J Cestero¹

¹Laboratory of Intracellular Bacterial Pathogens, National Centre for Biotechnology (CNB-CSIC), Madrid, Spain.

Infection and immunity

|January 2, 2025

概括

细菌酸糖合成酶,青素结合蛋白 (PBPs),直接影响第三类分泌系统 (T3SSs). PBP2对于SPI-1 T3SS的毒性至关重要,而其他PBP则增强了沙门氏菌中的SPI-2 T3SS活性.

科学领域:

微生物学微生物学
细菌病原体的产生
细胞壁合成细胞壁合成

背景情况:

第三种类型的蛋白质分泌系统 (T3SSs) 是格拉姆阴性细菌中必不可少的毒性因素,利用甘 (PG) 层作为结构支持.
沙门氏菌 Enteric Serovar Typhimurium 在宿主细胞入侵后,对病原体特定的青素结合蛋白 (PBPs),PBP2_SAL和PBP3_SAL进行升级调节,以响应环境线索.
在PG合成和T3SS调节之间的相互作用,特别是关于病原体特异性PBPs和毒性相关的T3SS (SPI-1和SPI-2),仍然不完全理解.

研究的目的:

调查参与类甘油合成的形态遗传性青素结合蛋白 (PBPs) 与沙门氏菌T3SSs.活动之间的功能关系.
为了确定PBP活动是否影响沙门氏菌Typhimurium中与毒性相关的SPI-1和SPI-2T3SS.

主要方法:

利用了Salmonella Typhimurium的同位素突变,缺少四种形态遗传PBP中的每一种.
评估了SPI-1和SPI-2T3SSs在这些PBP突变菌株中的活性.
通过检查SPI-1特定监管机构Hila和InvF的作用来调查监管机制.

主要成果:

PBP2对于SPI-1 T3SS的正常功能至关重要,但不需要用于细菌运动.
缺少四种形态基因PBP中的任何一种,会导致SPI-2 T3SS的活性增加.
PBP2通过Hila和InvF调节器积极调节SPI-1 T3SS.

结论:

这项研究提供了第一个直接将负责细菌细胞壁合成 (PBPs) 的酶与与毒性相关的T3SSss的调节联系起来的证据.
特定的PBP在调节不同T3SS的活性方面发挥着不同的作用,突出了新的细菌毒性控制层.
这些发现为细菌细胞包膜合成和感染期间蛋白质分泌机制的协调提供了新的见解.

关键词:

PBP2 PBP2是什么意思在PBP2SAL中使用.PBP3 PBP3 在线阅读在 PBP3SAL 中使用.沙门氏菌是一种沙门氏菌.第三种类型的分泌系统.

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