人类艾契病毒感染性从原始地表水到饮用水的持续性
Khira Sdiri-Loulizi1,2,3, Amira Khachou2, Stephanie Lemaire1,4
1Centre National de Référence des virus des gastro-entérites, Centre Hospitalier Universitaire Dijon Bourgogne, Dijon, France.
Applied and environmental microbiology
|January 2, 2025
概括
人类艾契病毒1 (AiV-1) 是胃肠炎的原因,在突尼斯的水样中经常检测到. 传染性AiV-1在水处理过程中持续存在,构成公共卫生风险,并突出显示了ICC-RT-qPCR对病毒监测的有用性.
科学领域:
- 环境微生物学 环境微生物学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 人类艾契病毒1 (AiV-1) 是一种通过水和食物传播的皮科纳病毒,引起胃肠炎.
- 在环境水域的高流行率表明AiV-1的潜力作为便污染指标.
- 了解AiV-1在水处理中的持久性对于公共卫生风险评估至关重要.
研究的目的:
- 选突尼斯的AiV-1表面和饮用水样本.
- 为了评估传染性AiV-1颗粒在整个水处理过程中的持久性.
- 评估综合细胞培养与定量分子检测 (ICC-RT-qPCR) 结合用于监测病毒风险的适用性.
主要方法:
- 使用实时逆转录酶PCR (RT-qPCR) 对450个水样 (表面,原始,处理) 的选.
- 使用ICC-RT-qPCR进行传染性颗粒持久性的评估.
- 病毒载荷的分析和感染性AiV-1基因型A的检测.
主要成果:
- 85%的水样对AiV-1呈阳性,病毒载量高达11.62 log10 cp/L.
- 在各种处理阶段的样本中检测到传染性AiV-1颗粒,包括原料,脱,花和处理过的水.
- 龙头水样本没有显示传染性AiV-1,但17.6%的阳性样本含有传染性颗粒.
结论:
- 传染性AiV-1在环境和经过处理的水中持久存在,构成了潜在的公共卫生威胁.
- AiV-1的存在强调了对水质强有力的监测的需要.
- ICC-RT-qPCR是一种实用和有效的工具,用于监测水中环境中人类的水源病毒风险.
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