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在猪PLA2G16中插入280bp的SINE可能会通过与其转录集成来修改基因表达

Cai Chen^1,2, Mengli Wang¹, Yao Zheng¹

¹College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Journal of applied genetics

|January 2, 2025

概括

在PLA2G16基因中的一个短间隔核元素逆转移子插入多态 (SINE-RIP) 影响脂质代谢. 这种SINE插入显示了对猪PLA2G16基因表达的组织特异性影响.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
动物科学动物科学

背景情况:

脂酶A2组XVI (PLA2G16) 基因在脂质代谢中起着至关重要的作用.
一种先前确定的短间隔核元素逆转移子插入多态 (SINE-RIP) 存在于PLA2G16基因的3'未翻译区域 (3'UTR).

研究的目的:

确认和分析PLA2G16基因中280bp的SINE插入在十种猪品种的分布.
为了研究SINE插入与PLA2G16.6的共同转录潜力.
以组织特定的方式评估SINE插入对PLA2G16基因表达的影响.

主要方法:

用聚合酶链反应 (PCR) 和测序来检测SINE插入及其分布.
使用逆转录PCR (RT-PCR) 来评估共转录.
使用定量PCR (qPCR) 来量化PLA2G16表达水平.

主要成果:

在十种猪品种中观察到显著的SINE插入多态性.
发现SINE插入与PLA2G16基因共同转录.
SINE插入对背部脂肪和肝脏中的PLA2G16表达产生了组织特异性影响.
与特定品种的SINE-/-猪相比,同卵性SINE插入 (SINE+/+) 猪在背部脂肪中显示出明显较低的PLA2G16表达 (p < 0.01) 和肝脏中的表达更高 (p < 0.05).

结论:

在PLA2G16 3'UTR中插入SINE可以通过共同转录形成一个新的转录.
这种新型的转录可能会以组织特定的方式影响PLA2G16基因表达,影响脂质代谢.
这些发现凸显了SINE插入作为影响猪基因表达和潜在的表型特征的遗传变异来源的作用.

关键词:

SINE; 3′UTR; 聚变的成绩单; PLA2G1616

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