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Updated: May 7, 2025

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Published on: June 6, 2019

通过光板显微镜和计算重建的谱系追踪.

Maria Kalogeridi^1,2, Ioannis Liaskas¹, John Rallis^1,2,3

¹Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Heraklion, Crete, Greece.

Methods in molecular biology (Clifton, N.J.)

|January 2, 2025

概括

现代实时成像,特别是光片光显微镜 (LSFM),允许详细的细胞谱系追踪. 这项研究详细介绍了使用开源软件 (MaMuT,Mastodon,TrackMate) 来重建Parhyale hawaiensis胚胎中的细胞发育.

关键词:

细胞谱系细胞谱系甲类的甲类动物斐济 - 斐济 - 斐济 - 斐济图像J 图像J 图像J 光片光显微镜光片光显微镜马姆·穆特 (马姆·穆特) 是一个马斯托登是一个巨.帕里亚尔夏威夷人 (Parhyale hawaiensis) 是一个夏威夷人.分段化分段化分段化分段化我们的TrackMate是我们的TrackMate.追踪追踪追踪追踪

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科学领域:

发展生物学发展生物学
显微镜技术显微镜技术
生物成像是一种生物成像.

背景情况:

像光片光显微镜 (LSFM) 这样的实时成像方法对于在体内可视化细胞动态至关重要.
LSFM提供高时空分辨率,最小的光损伤和整个样本的成像,显著地推进了血统追踪.
软件工具对于将原始图像数据转换为可量化的细胞系信息至关重要.

研究的目的:

描述LSFM图像数据集为谱系追踪的准备过程.
为了展示自由可用的软件平台 (MaMuT,Mastodon,TrackMate) 的应用,用于细胞和细胞核的跟踪.
为了说明类模型Parhyale hawaiensis的血统重建,用于发育和再生研究.

主要方法:

使用光片光显微镜 (LSFM) 进行高分辨率,长期成像的光标记胚胎.
使用斐济/ImageJ插件Massive Multiview Tracker (MaMuT),Mastodon和TrackMate进行细胞核或细胞细分和跟踪.
实施手动,半自动和全自动管道用于谱系重建.

主要成果:

成功准备了适合详细谱系分析的LSFM图像数据集.
证明了MaMuT,Mastodon和TrackMate在从LSFM数据中重建细胞系的有效性.
在Parhyale hawaiensis模型中对细胞行为和发育史进行定量分析.

结论:

自由可用的软件平台有效地支持使用LSFM数据进行小规模和大规模的血统追踪.
与先进的跟踪软件相结合的LSFM为研究发展和再生提供了一个强大的框架.
帕里亚尔夏威夷人模型非常适合使用这些成像和分析技术对细胞动态的多尺度调查.