Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ligand Binding Sites02:40

Ligand Binding Sites

12.7K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.7K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

DDX6 induces immunosuppression in cancer by disrupting structural stability of endogenous double-stranded RNAs.

Science immunology·2026
Same author

Iodide Anion Anchoring by Silver Nanoparticles Enables Shuttle-Free Zinc-Iodine Batteries.

Angewandte Chemie (International ed. in English)·2026
Same author

Connexin 43 promotes stemness of leukemia cells and chemoresistance in T-cell acute lymphoblastic leukemia via the RAC1/AKT/GSK3β axis.

Chinese medical journal·2026
Same author

Patient-reported outcomes in chronic GVHD: instruments, clinical utility, and survivorship integration.

Journal of patient-reported outcomes·2026
Same author

A thiadiazolylidene-morpholine compound inhibits Pseudomonas aeruginosa by destabilizing the thiamine monophosphate kinase thiL.

The Journal of biological chemistry·2026
Same author

CES1 deficiency is associated with metabolic reprograming and endothelial dysfunction in pulmonary arterial hypertension.

American journal of respiratory and critical care medicine·2026

相关实验视频

Updated: Jun 4, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.7K

高精度蛋白质结合的结构基础及其应用

Kelvin Han Chung Chong1,2, Lichao Liu3, Rae Chua2,4

  • 1School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 636921, Singapore.

Journal of the American Chemical Society
|January 2, 2025
PubMed
概括

研究人员设计了Connectase, 一种用于精确蛋白质结合的新型酶, 克服了现有方法的局限性. 在复杂的生物环境中进行高精度蛋白结合的修改.

更多相关视频

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
00:08

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.0K
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.4K

相关实验视频

Last Updated: Jun 4, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.7K
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
00:08

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.0K
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.4K

科学领域:

  • 生物化学
  • 结构生物学
  • 分子工程

背景情况:

  • 与化学方法相比,酶催化蛋白质修饰提供了更高的精度和兼容性.
  • 目前存在的酶如Sortase A和OaAEP1在目标特异性和复杂生物环境中的应用方面存在局限性.
  • 连接酶是一种重新设计的蛋白质酶,对蛋白质结合有希望,但缺乏可优化的过程性和结构数据.

研究的目的:

  • 通过X射线结晶学阐明MmConnectase (MmCET) 活动的结构基础.
  • 设计Connectase以提高蛋白质结合的精度和过程性.
  • 在具有挑战性的生物环境中实现高精度的蛋白质结合.

主要方法:

  • 在apo和基质结合状态下MmmConnectase的X射线晶体学.
  • 使用MjCET进行比较结构分析以确定关键的功能差异.
  • 针对N端基质识别动机的位点定向突变发生.
  • 单分子蛋白展开实验以评估结合效率和过程性.

主要成果:

  • 确定了MmConnectase的X射线晶体结构,揭示了其结合活性的结构基础.
  • 确定了MmConnectase与其不活跃的对应物MjCET的主要结构特征.
  • 建议并验证的修改抑制蛋白酶活性并提高结合精度.
  • 使用优化的Connectase和OaAEP1 ((C247A) 进行了逐步并联的证明,用于聚合物形成.

结论:

  • 对MmConnectase的结构洞察力有助于其进行先进的蛋白质结合工程.
  • 在复杂的生物环境中实现高精度的蛋白质结合,如细胞培养.
  • 优化Connectase显示了用于创建定义的蛋白质聚合物的增强过程性.