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在使用多重免疫组织化学的头和部状细胞癌中接受新辅助免疫疗法后,瘤免疫微环境的表征

Zhaohong An¹, Xiwei Zhang¹, Zhaoyang Wang¹

¹Department of Head and Neck Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

|January 2, 2025

概括

免疫细胞动态,包括T细胞和PD-1等检查点,预测头癌对新辅助免疫疗法的反应. 较低的TIM-3和LAG-3表达在治疗后表明更好的结果.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
癌症研究癌症研究

背景情况:

在头部和部状细胞癌 (HNSCC) 中,临床决策因新辅助免疫疗法 (NIT) 后免疫细胞动态和检查点调节不清楚而复杂.
了解这些变化对于优化治疗策略和预测患者反应至关重要.

研究的目的:

调查HNSCC患者在新辅助免疫疗法之前和之后的免疫细胞动态和免疫检查点表达.
在HNSCC中确定治疗反应的预测生物标志物.

主要方法:

在新辅助治疗前和后收集HNSCC活检样本.
样本被分为病理反应 (PR) 和非病理反应 (NPR) 组.
多重免疫组织化学 (m-IHC) 用于分析免疫细胞群 (CD4 +,CD8 + T 细胞,Treg) 和检查点表达 (PD-1,PD-L1,TIM-3,LAG-3).

主要成果:

在治疗前,PR患者的CD4 +,CD8 + T细胞,Treg,PD-1和PD-L1比NPR患者更高 (p<0.05).
在治疗后,大多数标志物在PR患者下降,与NPR患者相比,治疗前/治疗后比率较低.
在治疗后的PR组中,T细胞TIM-3+和LAG-3+显著降低 (p<0.05).
在PR组的瘤边缘区域中,CD8+ T细胞密度更高.

结论:

在HNSCC中,T细胞的存在是新辅助免疫疗法响应的重要预测因素.
治疗后T细胞TIM-3+和LAG-3+的较低水平与部分反应 (PR) 有关.
这项研究提供了对免疫细胞对HNSCC的新辅助免疫治疗反应生理变化的关键见解.

关键词:

头部和部状细胞癌多重复合免疫组织化学新辅助免疫疗法新辅助免疫疗法瘤免疫微环境

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