通过离心力改善细胞模式的组织和清晰度
Lauren E Mehanna1, James D Boyd2, Shelley Remus-Williams2
1Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, United States of America.
Biomedical materials (Bristol, England)
|January 2, 2025
概括
离心法有效地清洗图案细胞用于组织制造,去除未附着的细胞,同时保留所需的图案. 这种方法支持多种细胞类型和基板,增强高通量组织工程.
科学领域:
- 生物技术是生物技术.
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 高通量组织制造需要精确的细胞放置.
- 现有的粘合细胞造型方法在洗复杂性和统一性方面存在局限性.
- 离心法通常用于细胞粘附研究,而不是选择性模式.
研究的目的:
- 为了研究离心法作为一种洗细胞模式的方法,选择性粘附后.
- 评估离心法在创造有组织的细胞模式方面的有效性.
- 评估这种技术在不同细胞类型和基质的适应性.
主要方法:
- 使用生物素-斯特雷普塔维丁用于H9C2细胞的粘合细胞图案.
- 应用离心法来清洗有图案的细胞.
- 测试了各种线性结构 (50-200微米线宽) 的图案.
- 在模拟和洗后评估细胞活力.
- 对原I和Matrigel的MPC和HUVEC的评估管道形成指标.
主要成果:
- 离心法显著地从没有图案的区域中去除了细胞,同时保留了图案区域中的细胞.
- 已成功地实现了从50-200μm的线宽结构的模式.
- 在模式化和洗过程后,细胞活力保持在80%以上.
- 管形成指标在直接播种和离心模式细胞之间是可比的.
结论:
- 离心是一种可行且有效的方法,用于在组织制造中清洗细胞模式.
- 开发的模式和离心系统可以适应各种细胞系和生物材料.
- 这种技术为为各种生物应用创造量身定制的细胞模式提供了一个有前途的方法.
相关概念视频
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
Determining the Plane of Cell Division
3.3K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.3K
Cell Migration
4.7K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
4.7K
Subcellular Fractionation
6.8K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Differential Centrifugation
Differential centrifugation is...
6.8K
Centrifugation
2.1K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.1K
Centrioles and Centrosomes
2.6K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
Near the end of the prophase, also called late prophase or...
2.6K


