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通过基于表观遗传修饰的调节和Saccharomyces cerevisiae中的代谢工程改善了铁醇的生物合成

Guoli Wang¹, Xiqin Liang¹, Zhenke Wu¹

¹Featured Laboratory for Biosynthesis and Target Discovery of Active Components of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Binzhou Medical University, Yantai 264003, PR China.

Journal of biotechnology

|January 2, 2025

概括

这项研究利用表观遗传修饰和途径优化设计了一种微生物菌株,用于高效的铁醇生物合成. 改造品种的铁醇产量增加了61.7倍,达到954.69 mg/L.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

芳香氨基酸和衍生品是有价值的化学物质,目前的生产方法有局限性.
对于工业应用而言,像醇这样的化合物的高效生物合成至关重要.

研究的目的:

开发一种有效的生物合成途径,用于生产醇.
利用基于表观遗传修饰的调节来增强芳香氨基酸化合物生物合成.

主要方法:

过度表达m6A修改写字器Ime4和阅读器Pho92,以及调节器Gcr2.2.
遗传修饰包括Bbxfpk的引入,Gpp1的删除,以及通过Aro4K229L和Aro7G141S突变的反抑制缓解.
通过删除PHA2,取代ARO10促进体,并引入氨酸脱碳酶PcAAS来构建最终菌株.

主要成果:

通过表观遗传调节剂的过度表达和途径优化,提洛产生的显著改善.
通过引入Bbxfpk和删除Gpp1.1,进一步提高了铁醇产量.
达到最终的铁醇度为954.69 ± 43.72 mg/L,增加了61.7倍.

结论:

基于表观遗传修饰的调节与代谢工程策略相结合,可以显著增强铁醇生物合成.
开发的工程菌株为工业化生产铁醇提供了一个有前途的平台.
这项研究表明了将表观遗传调节整合到微生物细胞工厂中,用于高价值化学品生产的潜力.

关键词:

芳香氨基酸是一种芳香氨基酸.代谢工程是代谢工程.这种植物是Saccharomyces cerevisiae.m(6) 一个修改修改.

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