相关实验视频

Updated: Jun 4, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

利用合成抗体-DNA结合物来扩展CRISPR-Cas12a生物传感工具箱

Elisa Paialunga¹, Neda Bagheri¹, Marianna Rossetti¹

¹Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy.

ACS synthetic biology

|January 2, 2025

概括

抗体-DNA结合体激活CRISPR-Cas12a,以进行敏感的检测. 这种新的CRISPR技术使得针对SARS-CoV-2尖端蛋白等点的快速,高度敏感的免疫试验成为可能.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
免疫技术是一种免疫技术.

背景情况:

克里斯普尔-Cas12a表现出附带分裂活动,通常由核酸点激活.
免疫测试通常使用酶标签来生成信号,但其灵敏度和速度可能受到限制.
抗体-DNA合物提供了抗体识别和基于核酸的检测系统之间的潜在桥梁.

研究的目的:

研究使用抗体-DNA合物 (Ab-DNA) 来激活CRISPR-Cas12a附带活性.
开发基于CRISPR的新型免疫试验,用于检测非核酸标.
将这些新型测试与传统免疫测试方法的性能进行比较.

主要方法:

抗体与DNA链的结合产生Ab-DNA激活剂.
使用Ab-DNA结合体触发CRISPR-Cas12a附带裂变的过程.
基于CRISPR的免疫传感测试 (CIA) 和基于CRISPR的免疫磁性测试 (CIMA) 的开发.
使用CIA和CIMA检测SARS-CoV-2尖端S蛋白.

主要成果:

Ab-DNA结合物成功地激活了CRISPR-Cas12a.的附带分裂活动.
与ELISA相比,中央情报局对SARS-CoV-2尖端S蛋白检测表现出更高的灵敏度.
在2小时内,CIMA在未稀释的唾液中实现了SARS-CoV-2尖端S蛋白的890 pM检测极限.

结论:

Ab-DNA结合物有效地将抗体识别与CRISPR-Cas12a激活联系起来,使光输出成为可能.
基于CRISPR的免疫试验 (CIA和CIMA) 为传统免疫试验提供了灵敏和快速的替代方案.
这种方法为使用CRISPR技术检测非核酸点提供了一个多功能平台.

关键词:

这就是CRISPR-Cas12a.抗体DNA结合物抵押品的分离,就是抵押品的分离.光是一种光.免疫测试是一种免疫测试.跨裂纹的跨裂纹

更多相关视频

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...