单分子双色和三色FRET研究揭示了NSF在SNARE拆卸中的过渡状态
Sudheer K Cheppali1, Chang Li1, Wenjing Xing1
1State Key Laboratory of Membrane Biology, Beijing Frontier Research Center of Biological Structure, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Nature communications
|January 2, 2025
概括
可溶性N-乙基胺敏感因子附着蛋白受体 (SNARE) 复合体由N-乙基胺敏感因子 (NSF) 分解,通过两个不同的途径发生. 这些途径涉及连续的蛋白质去除,揭示了以前没有观察到的合成素分离的中间状态.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- SNARE蛋白质调解囊泡融合,这是真核细胞中的一个关键过程.
- 通过N-乙烯胺胺敏感因子 (NSF) 拆解SNARE复合物对于膜融合回收至关重要.
- 一个AAA+ ATPase的NSF与像α-SNAP这样的适配蛋白一起起作用,以拆解SNARE复合体.
研究的目的:
- 为了阐明NSF在SNARE复杂拆卸过程中发生的连续事件.
- 调查NSF在SNARE机械回收中的作用.
- 描述SNARE复杂分解过程中所涉及的中间状态.
主要方法:
- 单分子光光谱学.
- 生物物理技术用于监测蛋白质相互作用和动态.
- 在SNARE复合物的体外复合和拆卸试验.
主要成果:
- 通过NSF识别了SNARE复合体拆卸的两个不同的顺序路径.
- 在拆卸过程中观察到一种新的合成素分离的中间状态.
- 证明分解可以通过合成素或Vamp解离来启动.
结论:
- 通过NSF进行SNARE复杂拆卸是一个通过并行路径发生的多步骤过程.
- 鉴定合成素分离的中间体为分解机制提供了新的见解.
- 了解SNARE拆卸对于理解膜贩运及其监管至关重要.
相关概念视频
01:26Fusion of Secretory Vesicles with the Plasma Membrane
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
01:43SNAREs and Membrane Fusion
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...