相关实验视频

Updated: Apr 29, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

染色体层次的基因组组合的Monolepta 象形形状,两斑叶甲虫 (Coleoptera: Chrysomelidae) 的

Hao He¹, Yongxin Zhao¹, Dengyu Zheng¹

¹Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Scientific data

|January 2, 2025

概括

为农业害虫Monolepta hieroglyphica创建了一个高质量的基因组组. 这个资源有助于理解甲虫.

科学领域:

昆虫学昆虫学是一门学科.
基因组学就是基因组学.
农业科学农业科学

背景情况:

象形字形虫 (Monolepta hieroglyphica) 是东亚和东南亚的重要农业害虫,原因是它拥有广泛的宿主范围和多性质.
了解其遗传构成对于开发有效的害虫管理策略至关重要.

研究的目的:

为了生成一个高质量的,染色体级的基因组组合为Monolepta hieroglyphica.
为研究甲虫生物学和作物保护提供有价值的分子资源.

主要方法:

使用Hi-C数据进行染色体级基因组组装.
使用综合方法进行基因预测.
使用BUSCO.CO进行基因组完整性评估.

主要成果:

实现了具有18.62 Mb的contig N50和241.916 Mb的N50支架的基因组组装.
91.9%的基因组固定在九个染色体上.
预测了46 561个基因,具有很高的完整性 (99.4%的基因组,95.2%的蛋白质组).

结论:

基因组组合的染色体水平的Monolepta 象形文字是一个显著的进步.
这一资源将促进未来关于甲虫遗传学,进化和新型控制方法的研究.

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