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通过低温电子显微镜揭示了可溶性α-klotho的形态景观

Nicholas J Schnicker^1,2, Zhen Xu³, Mohammad Amir⁴

¹Protein and Crystallography Facility, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA. nicholas-schnicker@uiowa.edu.

Scientific reports

|January 2, 2025

概括

可溶性α-Klotho (sKLA) 具有独立于FGF23信号传输的多种功能. 结构和生物物理研究揭示了sKLALA的存在.

科学领域:

生物化学生化学
结构生物学结构生物学
分子内分泌学分子内分泌学

背景情况:

α-Klotho (KLA) 是一种与纤维细胞生长因子受体 (FGFR) 和FGF23.3相关的蛋白质.
可溶性KLA (sKLA) 具有独立于FGFR/FGF23信号传输的功能.
之前的结构表明,sKLA仅作为FGF23.23的核心受体.

研究的目的:

为了研究apo-sKLA的生物物理性质和结构.
了解sKLA的FGFR/FGF23独立功能.

主要方法:

单颗粒冷电子显微镜 (cryo-EM) 用于结构确定.
质量摄影仪用于分析分子相互作用.
用于结合部位分析的计算研究.

主要成果:

3.3 Å分辨率的apo-sKLA的冷电磁结构揭示了KL2领域的独特特征和灵活性.
sKLA采用多种形状,具有不同的域间角度.
质量摄影仪和冷EM证实SKLA可以与FGFR和/或FGF23.3形成二次体和稳定复合体.
计算分析表明,FGF23的两个KLA结合位点可以在二次体中结合一个单独的sKLA分子.

结论:

结构性可塑性和二元化潜力支持sKLA作为具有类作用的独立激素的作用.
FGF23与两个sKLA分子同时接触,表明一种新的信号传递机制.

关键词:

三维可变性分析 (3DVA)低温电子显微镜 (cryo-EM) 是一种低温电子显微镜.迪默 (Dimer) 是一个数字.纤维细胞生长因子 (FGF)一个单体的单体.可溶性α-克洛托 (sKLA) 是一种可溶性α-克洛托.一个三角形复合体.

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