在堕胎胎儿的羊水四分学中分析外体miRNA概况
Hainan Yang1,2, Yadan Li3, Qiuyue Chen2
1Department of Ultrasound, First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361003, Fujian, China.
Scientific reports
|January 3, 2025
概括
羊水外体微RNA与Fallot (TOF) 的四分法有关. 这些外体体中的MiR-10a-5p通过准TBX5来损害心脏发育,从而导致TOF的发病.
科学领域:
- 心血管生物学 心血管生物学
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
背景情况:
- 胚胎液 (AF) 衍生的外体微RNA (miRNA) 越来越多地被认为对胎儿发育和疾病发病的作用.
- 费洛特四重症 (TOF) 是一种复杂的先天性心脏缺陷,其分子机制尚未完全理解.
- 在AF中的外体miRNA可能为TOF病因提供了洞察力.
研究的目的:
- 在TOF的胎儿中研究AF衍生的外体miRNA的表达特征.
- 为了确定有助于TOF发展的特定miRNAs.
- 阐明AF衍生的外体miRNAs在TOF病变发生过程中的作用背后的分子机制.
主要方法:
- 从有TOF胎儿的孕妇和健康对照的AF中分离出外体.
- 使用Agilent人类miRNA阵列V21.0.0进行了miRNA表达概况.
- 使用了生物信息分析 (KEGG通路,双露西法酶记者试验) 和细胞分化试验 (P19细胞).
主要成果:
- 不同表达分析揭示了TOF样本中257个显著失调的miRNA.
- 丰富的途径包括与先天性疾病相关的途径,其中25个miRNA与Notch和Wnt信号相关.
- 过度表达miR-10a-5p通过直接向TBX5.5来抑制P19细胞中的心肌原体分化.
结论:
- 由AF衍生的外体miR-10a-5p通过损害心肌细胞分化来促进TOF的病变发生.
- 这种miR-10a-5p/TBX5相互作用代表了TOF发育中的新型分子机制.
- 这些发现为TOF的分子基础提供了更深入的理解.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...


