埃纳洛米克:一种基于质谱的方法,用于在生物样本中分析,识别和半量化埃纳尔
Congyu Ma1, Wen Zhang2, Jialong Jing1
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P. R. China.
Analytical chemistry
|January 3, 2025
概括
研究人员开发了"enalomics",一种质谱法,用于识别和量化称为enals的活性化物. 这种方法确定了心肌梗塞的新指标和潜在生物标志物,有助于诊断疾病.
科学领域:
- 生物化学和分子生物学
- 分析化学 分析化学
- 病理学 病理学 病理学
背景情况:
- 脂质过氧化 (LPO) 的化物影响细胞过程,并与疾病有关.
- 乙醇,反应性基代谢产物,与神经退行,癌症和心血管疾病有关.
- 内源性类由于不稳定性和低丰度而具有较差的特征.
研究的目的:
- 引入和验证基于质谱学的方法",enalomics",用于分析内源性enal.
- 在生物样本中识别和半量化.
- 评估ENAL在心肌损伤和心脏病发作中的诊断潜力.
主要方法:
- 开发了使用质谱法 (MS) 的"enalomics"与衍生 (2,4-dinitrophenylhydrazine) 和酸.
- 采用前体离子扫描,动态多重反应监测和高分辨率MS用于完全识别.
- 利用保留时间和碳素数相关性来进行可靠的识别和量化.
主要成果:
- 在老鼠组织中鉴定了157个 (127个新型) 和在人体血中鉴定了29个 (24个新型).
- 在患有心肌损伤的老鼠中,证明了内代谢的改变.
- 确定了14种用于心肌梗塞 (MI) 的血内生物标志物,具有高诊断准确性 (AUC ≥ 0.8603).
结论:
- 埃纳洛米克提供了一种全面的方法来分析生物系统中的内源性埃纳尔.
- 在心肌损伤和心脏病发作时,内代谢发生显著变化.
- 血内显示为早期诊断和干预心脏病发作患者的敏感生物标志物具有前途.
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