评估山羊和周围的水源感染的患病率,风险因素和诊断技术
Manahil Rafiq1, Naimat Ullah Khan2, Imad Khan2
1Department of Zoology, Abdul Wali Khan University, Mardan, Pakistan.
Frontiers in veterinary science
|January 3, 2025
概括
经过修改的Ziehl-Neelsen (ZN) 和Kenyon's Acid-Fast (KAF) 染色方法可以有效地检测山羊便中的Cryptosporidium. 然而,ZN染色显示出在水样中检测Cryptosporidium卵囊的卓越性能,这对于公共卫生监测至关重要.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 公共卫生 公共卫生
- 微生物学 微生物学
背景情况:
- 克里普托斯波里迪亚 spp. 它们是原生虫寄生虫,在像山羊这样的动物中引起胃肠道感染,并通过水污染构成公共卫生风险.
- 在便和水样本中精确检测Cryptosporidium卵囊对于流行病学研究和控制密菌病至关重要.
- 修改的Ziehl-Neelsen (ZN) 和肯昂的酸速 (KAF) 染色是卵囊识别的常用方法.
研究的目的:
- 为了比较ZN和KAF染色方法在山羊便和水样中检测Cryptosporidium卵囊的有效性.
- 评估这些方法在不同地理区域,性别,年龄组,腹状况和山羊养方案中的表现.
主要方法:
- 在巴基斯坦马丹的四个地理区域收集了300只山羊的便和300个水样.
- 根据动物的性别,年龄 (<1,1-2,>2岁),腹状态和养方式 (养,放牧) 分类样本.
- 将ZN和KAF染色技术应用于所有样本,并对检测率进行统计分析.
主要成果:
- 对于山羊便的总体检测率与ZN (61.00%) 和KAF (63.33%) 方法相似 (p > 0.05).
- 在腹山羊和特定地理区域 (Tehsil Katlang) 观察到更高的检测率.
- 对于水样,ZN染色与KAF染色 (1.00%) 相比,显著提高了检测率 (16.00%) (p < 0.001).
结论:
- 采用ZN和KAF两种染色方法,都能有效地检测山羊便中的Cryptosporidium.
- 采用ZN染色方法,可以在环境水样中检测出Cryptosporidium卵囊的灵敏度更高.
- 建议在环境监测中进行ZN染色,以减轻动物病性密码化症风险.
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