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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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相关实验视频

Updated: May 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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功能性核酸SERS传感策略的进展

Lin Shi1,2,3, Yukang Liu1,3, Xiaodong Li1,3

  • 1Center for Biomedical-photonics and Molecular Imaging, Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China.

ACS sensors
|January 3, 2025
PubMed
概括

功能性核酸,如DNAzymes和aptamers,可以通过表面增强的拉曼光谱法 (SERS) 进行超敏感检测. 本综述探讨了它们的设计和在诊断和环境监测中的应用.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这就是DNAzyme DNA酶.在G-quadruplex中使用.一个aptamer的应用程序.环境监测 环境监测 环境监测食品安全 食品安全功能性核酸 功能性核酸液体活检活检液体活检传感应用程序的应用程序.表面增强的拉曼光谱 (SERS)

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Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
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科学领域:

  • 生物化学 生化学
  • 分析化学 分析化学
  • 纳米技术 纳米技术

背景情况:

  • 功能性核酸表现出独特的结构,超出了传统的DNA放大.
  • 这些分子通过Hoogsteen键形成复杂的混合结构.
  • 基于功能性核酸的表面增强拉曼光谱 (SERS) 提供超敏感和多重检测能力.

研究的目的:

  • 审查功能核酸在SERS检测中的原理和应用.
  • 阐明基于核酸的功能SERS传感器的设计策略.
  • 突出疾病诊断,环境监测和食品安全方面的进展.

主要方法:

  • 功能性核酸的结构属性和SERS感知机制的概述 (DNA酶,G-四复合体,体,CRISPR,原形).
  • 详细说明了构建功能性核酸基SERS传感器的创新策略.
  • 传感器应用的综合总结,重点关注灵敏度,特异性和多功能性.

主要成果:

  • 功能性核酸可通过SERS进行高度敏感和特定的分析物检测.
  • 不同的功能性核酸表现出不同的传感机制和应用.
  • 创新的传感器设计提高了关键领域的检测能力.

结论:

  • 基于功能性核酸的SERS是一种强大的超敏感检测平台.
  • 在疾病诊断,环境监测和食品安全方面取得了重大进展.
  • 未来的研究应该解决现有挑战,并探索增强分析性能的新途径.