欧巴托林通过抑制NF-κB/P13K/AKT信号通路来调节甲状腺癌细胞增殖中的BCPAP
Weiqi Song1, Rongyue Yao2, Annamalai Vijayalakshmi3
1Department of Head and Neck Oncology, Shaanxi Provincial Cancer Hospital Affiliated to Xi'an Jiaotong University, China.
概括
欧巴托林 (EUP) 通过向关键信号通路,有效抑制乳头甲状腺癌 (PTC) 细胞生长,并诱导细胞亡. 这种天然的黄为治疗这种常见的内分泌癌症提供了有前途的治疗策略.
科学领域:
- 内分泌学 在内分泌学.
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 甲状腺癌 (TC) 是全球最常见的内分泌癌,与分化的亚型,如乳头甲状腺癌 (PTC) 是普遍的,特别是在妇女中.
- 尽管有可用的治疗方法,TC仍然难以治愈.
- 欧巴托林 (EUP),一种天然的黄,已显示出抗癌性质,但其针对PTC的特定机制尚不清楚.
研究的目的:
- 研究eupatorin (EUP) 对人类PTC细胞 (BCPAP) 的抗增殖和亡作用.
- 阐明EUP在PTC中的作用的基础分子机制.
主要方法:
- 使用MTT测定,AO/EB,Rh-123和DAPI染色来评估细胞活力和细胞亡.
- 进行了粘附测试和西部斑分析,以评估蛋白质表达.
- 研究了EUP对BCPAP细胞的增殖和亡的影响,度为20和30μM/mL.
主要成果:
- 在剂量取决的方式中,EUP显著抑制了BCPAP细胞的增殖.
- 通过上调caspase-9和p53的表达,EUP诱导了亡,同时降低了增殖细胞核抗原 (PCNA) 水平.
- 通过抑制核因子kappa B (NF-κB) 信号传递,EUP减少了炎症和BCPAP的扩散,这反过来影响了P13K/AKT信号传递.
结论:
- 欧巴托林在人类PTC细胞上表现出强大的抗增殖和亲产的作用.
- 欧欧通过抑制NF-κB和P13K/AKT信号通路来发挥其抗癌作用.
- 这些发现表明EUP是乳头甲状腺癌的潜在治疗剂.
相关概念视频
Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists
82
Endothelins (ETs) are potent vasoactive peptides critical in the human body's various physiological and pathological processes. One of the most promising therapeutic strategies for treating pulmonary arterial hypertension (PAH) involves counteracting the effects of these endothelins using a class of drugs known as endothelin receptor antagonists.
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme...
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme...
82
Mitogens and the Cell Cycle
6.3K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.3K
NF-κB-dependent Signaling Pathway
7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.2K
Interactions Between Signaling Pathways
6.1K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.1K
Treatment for Pulmonary Arterial Hypertension: Receptor Tyrosine Kinase Inhibitors and Calcium Channel Blockers
89
Receptor tyrosine kinase inhibitors (TKIs) and calcium channel blockers (CCBs) are two critical categories of drugs employed in the treatment of pulmonary artery hypertension (PAH). PAH is a disease that causes high blood pressure in the pulmonary arteries, resulting in chest pain, fatigue, and shortness of breath.
TKIs, such as imatinib (Gleevec), are particularly effective in tackling the growth and mitogenic factors that become upregulated in PAH patients. These factors contribute to the...
TKIs, such as imatinib (Gleevec), are particularly effective in tackling the growth and mitogenic factors that become upregulated in PAH patients. These factors contribute to the...
89
Transducer Mechanism: Enzyme-Linked Receptors
2.3K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
2.3K


